Overview

  • Product name
  • Description
    Rabbit polyclonal to SCD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Guinea pig, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: HVGWLLVRKH PAVKEKGSTL DLSDLEAEKL VMFQRRYYKP GLLMMCFILP, corresponding to internal sequence amino acids 181-230 of Human SCD

  • Positive control
    • Fetal liver lysate Human liver tissue slide

Applications

Our Abpromise guarantee covers the use of ab23331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Function
    Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • Sequence similarities
    Belongs to the fatty acid desaturase family.
  • Domain
    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

Images

  • Anti-SCD1 antibody (ab23331) at 1 µg/ml + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SCD1 with ab23331 at a concentration of 4-8µg/ml. Cells with positive staining are marked by arrows. Magnification: X400.

References

This product has been referenced in:
See all 2 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (PAGE 12%)
Sample
Cow Cell lysate - whole cell (bovine fetal liver lysate)
Specification
bovine fetal liver lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 09 2014

Question
Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab23331 has never been tested in ELISA so we do not know whether or not it is suitable for this application. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

It looks like you may have already purchased this antibody, so if you decided to test it in ELISAwe woulencourage you to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

Read More

Answer

Thank you for contacting us.

According to SwissProt, the predicted MW of the SCD human protein is 41.5 kDa.

http://www.uniprot.org/uniprot/O00767

We made a note on the datasheet to point out that the band observed at 37 kDa is a bit lower than the predicted MW of the protein mass, and this may be due to splicing.

If you're observing a band at 70-80 kDa, a few things could be going on. Firstly, it may be a dimer that is forming. Are you boiling the samples for 5 min? Are the conditions reduced and denatured (with SDS)? What is the lysis buffer that you're using?

Since this is a transmembrane protein, these can sometimes have difficulty running in the gel and can aggregate with boiling. We recommend heating to 70 C for 5-10 mins to allow them to enter the gel more efficiently.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

DISCOUNT CODE: ***
Expiration date: September 11th, 2012

I am very pleased to hear you would like to accept our offer and test ab23331 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the "Additional Comments" section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (liver microsomes)
Loading amount
10 µg
Specification
liver microsomes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C

Dr. Colin Shearn

Verified customer

Submitted Jan 27 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab23331.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is notcovered by our Abpromise guarantee. Therefore, I am issuing you a testing discount code for testing this replacement antibody in mouse samples. Please see below for details.

I wish you the best of luck with your research.

===== TESTING DISCOUNT CODE =====

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23331 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

The peptide used was derived from that region - residues 184-238 of Human SCD. The antibody has not been tested against the SCD2 or SCD5 isoform. Please contact us again if you have any additional questions.

Read More

Answer

I was informed that the following method was used with ab23331 immunostaining of human liver sections: 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethanol for 1 minute each. 3. Rinse the sections in distilled water followed by 0.1 M PBS. 4. Quench endogenous peroxidase for 10 min with 3% H2O2 in 0.1 M PBS and wash 3 times with distilled water, 2 minutes each. 5. Retrieve epitopes by incubating the sections at 98-100° C in a microwave in 0.01 M citrate buffer (pH 6.0) for 10 minutes. Cool down in room temperature for 30-60 min. 6. Rinse the sections in 0.1 M PBS for 3 × 2 min. 7. Block the sections with 10% normal goat serum in 0.1 M PBS at room temperature for 30 min. 8. Incubate the sections with primary antibody at appropriate dilution in 0.1 M PBS overnight at 4°C. 9. Rinse the sections in 0.1 M PBS for 3 × 5 min. 10. Incubate the sections with biotinylated goat anti-rabbit IgG 11. Rinse the sections in 0.1 M PBS for 3 × 5 min. 12. Incubate the sections with HRP-conjugated streptavidin SPN-9001, Vector, 1/200 in 0.1 M PBS, 37 ° C, 20 min) 13. Rinse the sections with 0.1 M PBS for 3 × 5 min followed by 50 mM Tris-HCl buffer (pH 7.6). 14. Incubate with a peroxidase substrate solution. 15. Rinse the sections in distilled water. 16. Counterstain for 10-30 seconds with a hematoxylin counterstaining solution. 17. Rinse the sections with tap water. 18. Dehydrate through 70-80-90-95-100-100% of ethanols, each 3 minutes. 19. Clear in xylene for 2×3 min. 20. Coverslip with mounting medium. If you still experience problems with this protocol please do not hesitate to contact me again and I can offer you a refund or replacement vial if the antibody was purchased in the last 90days,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up