Recombinant
Anti-SCF antibody [EP665Y] (ab52603)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-SCF antibody [EP665Y]
See all SCF primary antibodies -
DescriptionRabbit monoclonal [EP665Y] to SCF
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human SCF aa 50-150 (N terminal). The exact sequence is proprietary.
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Positive control
- Human kidney carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP665Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab52603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Predicted molecular weight: 31 kDa. | |
| Flow Cyt | 1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/500. |
Target
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FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
Sequence similaritiesBelongs to the SCF family.
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Developmental stageActs in the early stages of hematopoiesis.
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Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
- Information by UniProt
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Database links
- Entrez Gene: 4254 Human
- Omim: 184745 Human
- SwissProt: P21583 Human
- Unigene: 1048 Human
- Unigene: 661108 Human
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Alternative names
- C kit ligand antibody
- C-kit ligand antibody
- Ckit ligand antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-263473-ab52603-MCF7methanol500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (ab52603)Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
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![Western blot - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603wb.jpg)
Western blot - Anti-SCF antibody [EP665Y] (ab52603)Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (ab52603)Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250. -
![Flow Cytometry - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/SCF-Primary-antibodies-ab52603-1.jpg)
Flow Cytometry - Anti-SCF antibody [EP665Y] (ab52603)Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Datasheets and documents
References
This product has been referenced in:
- Yong KS et al. Human CD34(lo)CD133(lo) fetal liver cells support the expansion of human CD34(hi)CD133(hi) hematopoietic stem cells. Cell Mol Immunol 13:605-14 (2016). ICC/IF . Read more (PubMed: 27593483) »
- Huang X et al. Three-dimensional co-culture of mesenchymal stromal cells and differentiated osteoblasts on human bio-derived bone scaffolds supports active multi-lineage hematopoiesis in vitro: Functional implication of the biomimetic HSC niche. Int J Mol Med 38:1141-51 (2016). WB . Read more (PubMed: 27571775) »
