Recombinant Anti-SCF antibody [EP665Y] (ab52603)
![Western blot - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-64933-anti-scf-antibody-ep665y-western-blot.jpg "Western blot - Anti-SCF antibody [EP665Y] (ab52603)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP665Y] to SCF
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-SCF antibody [EP665Y]
See all SCF primary antibodies -
Description
Rabbit monoclonal [EP665Y] to SCF -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human SCF aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P21583 -
Positive control
- Human kidney carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP665Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (3) |
1/10000. Predicted molecular weight: 31 kDa.
|
| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF | (2) |
1/100 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000. Predicted molecular weight: 31 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/500. |
Target
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Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. -
Involvement in disease
Hyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
Sequence similarities
Belongs to the SCF family. -
Developmental stage
Acts in the early stages of hematopoiesis. -
Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
Cellular localization
Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. - Information by UniProt
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Database links
- Entrez Gene: 4254 Human
- Omim: 184745 Human
- SwissProt: P21583 Human
- Unigene: 1048 Human
- Unigene: 661108 Human
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Alternative names
- C kit ligand antibody
- C-kit ligand antibody
- Ckit ligand antibody
see all
Images
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Western blot - Anti-SCF antibody [EP665Y] (ab52603)Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-263473-anti-scf-antibody-ep665y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (ab52603)Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-64932-anti-scf-antibody-ep665y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (ab52603)Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-64931-anti-scf-antibody-ep665y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] (ab52603)Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Anti-SCF antibody [EP665Y] (ab52603)](https://www.abcam.com/ps/products/52/ab52603/Images/ab52603-5-benefits-of-recombinant-antibodies.png)
Anti-SCF antibody [EP665Y] (ab52603)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (17)
ab52603 has been referenced in 17 publications.
- Su B et al. Apatinib exhibits synergistic effect with pyrotinib and reverses acquired pyrotinib resistance in HER2-positive gastric cancer via stem cell factor/c-kit signaling and its downstream pathways. Gastric Cancer 24:352-367 (2021). PubMed: 33030616
- Fang W et al. Molecular Hydrogen Protects Human Melanocytes from Oxidative Stress by Activating Nrf2 Signaling. J Invest Dermatol 140:2230-2241.e9 (2020). PubMed: 32234461
- Gavriilidis GI et al. Stem cell factor Is implicated In microenvironmental interactions and cellular dynamics of chronic lymphocytic leukemia. Haematologica N/A:N/A (2020). WB, IHC, Flow Cyt ; Human . PubMed: 32336682
- Hsiao CH et al. Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm. Stem Cell Res Ther 10:270 (2019). PubMed: 31445515
- Frumento G et al. CD117 (c-Kit) Is Expressed During CD8+ T Cell Priming and Stratifies Sensitivity to Apoptosis According to Strength of TCR Engagement. Front Immunol 10:468 (2019). PubMed: 30930902
