Recombinant Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
![Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-407887-anti-scf-antibody-ep665y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-SCF antibody [EP665Y] - BSA and Azide free
See all SCF primary antibodies -
Description
Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247320 is the carrier-free version of ab52603.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP665Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
|
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| WB |
1/10000. Predicted molecular weight: 31 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
Use at an assay dependent concentration. |
|
WB
1/10000. Predicted molecular weight: 31 kDa. |
Target
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Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. -
Involvement in disease
Hyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
Sequence similarities
Belongs to the SCF family. -
Developmental stage
Acts in the early stages of hematopoiesis. -
Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
Cellular localization
Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. - Information by UniProt
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Database links
- Entrez Gene: 4254 Human
- Omim: 184745 Human
- SwissProt: P21583 Human
- Unigene: 1048 Human
- Unigene: 661108 Human
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Alternative names
- C kit ligand antibody
- C-kit ligand antibody
- Ckit ligand antibody
see all
Images
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Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab52603, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-3-anti-scf-antibody-ep665y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-2-anti-scf-antibody-ep665y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-1-anti-scf-antibody-ep665y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-5-benefits-of-recombinant-antibodies.png)
Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247320 has not yet been referenced specifically in any publications.
