Anti-SCG10 antibody (ab21190)
Key features and details
- Goat polyclonal to SCG10
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCG10 antibody
See all SCG10 primary antibodies -
Description
Goat polyclonal to SCG10 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SCG10 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q93045 -
Positive control
- Human Brain lysate, A431 lysate ICC/IF: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa.
A 1 hour primary incubation is recommended for this product. |
Notes |
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ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa. A 1 hour primary incubation is recommended for this product. |
Target
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Function
Is a key regulator of neurite extension through regulation of microtubule instabilily. -
Tissue specificity
Neuron specific. -
Sequence similarities
Belongs to the stathmin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms. - Information by UniProt
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Database links
- Entrez Gene: 11075 Human
- Omim: 600621 Human
- SwissProt: Q93045 Human
- Unigene: 521651 Human
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Alternative names
- Neuron specific growth associated protein antibody
- Neuronal growth associated protein antibody
- Neuronal growth associated protein (silencer element) antibody
see all
Images
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Ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Anti-SCG10 antibody (ab21190) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 20.8 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab21190 has been referenced in 2 publications.
- Davis EJ et al. Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion. Neuroscience 146:784-91 (2007). PubMed: 17331653
- Ohkawa N et al. The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. J Cell Sci 120:1447-56 (2007). PubMed: 17389683