Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15286-39] to SCG10
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-SCG10 antibody [EPR15286-39]
See all SCG10 primary antibodies
DescriptionRabbit monoclonal [EPR15286-39] to SCG10
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human SCG10 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q93045
- Human fetal brain, SH-SY5Y and rat brain lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185956 in the following tested applications.
|WB||1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|IP||1/50 - 1/70.|
FunctionIs a key regulator of neurite extension through regulation of microtubule instabilily.
Tissue specificityNeuron specific.
Sequence similaritiesBelongs to the stathmin family.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms.
- Information by UniProt
- Neuron specific growth associated protein antibody
- Neuronal growth associated protein antibody
- Neuronal growth associated protein (silencer element) antibody
All lanes : Anti-SCG10 antibody [EPR15286-39] (ab185956) at 1/20000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Immunoprecipitation of Human fetal brain lysates using ab185956 (lane 1). Detection of SCG10 utilised ab185956 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. The control utilised PBS instead of Human fetal brain lysates (lane 2).
Based on the sequence analysis, ab185956 recognizes two isoforms with the predicted MWs of 21KDa and 22KDa, respectively.
ab185956 has not yet been referenced specifically in any publications.