Overview

  • Product name

    Anti-SCGB3A2 antibody [EPR11463]
    See all SCGB3A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11463] to SCGB3A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SCGB3A2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96PL1

  • Positive control

    • Calu 3 cell lysate; Human SCGB3A2 recombinant protein; A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11463
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
ICC/IF 1/500.

Target

  • Tissue specificity

    Highly expressed in lung.
  • Sequence similarities

    Belongs to the secretoglobin family. UGRP subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • LU103 antibody
    • Pneumo secretory protein 1 antibody
    • PnSP-1 antibody
    • PNSP1 antibody
    • SCGB3A2 antibody
    • Secretoglobin family 3A member 2 antibody
    • SG3A2_HUMAN antibody
    • UGRP1 antibody
    • Uteroglobin-related protein 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) labelling SCGB3A2 with purified ab181853 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunofluorescent analysis of A549 cells (paraformaldehyde-fixed, 4%) labeling SCGB3A2 with ab181853 at 1/500 dilution followed by Goat anti rabbit IgG Alexa Fluor 555) secondary at 1/200 dilution and counter-stained with DAPI.

  • Anti-SCGB3A2 antibody [EPR11463] (ab181853) at 1/1000 dilution + Human SCGB3A2 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 10 kDa

  • Anti-SCGB3A2 antibody [EPR11463] (ab181853) at 1/1000 dilution + Calu 3 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 10 kDa

References

ab181853 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181853.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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