Key features and details
- Mouse monoclonal [CL0271] to SCGN/Secretagogin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SCGN/Secretagogin antibody [CL0271]
See all SCGN/Secretagogin primary antibodies
DescriptionMouse monoclonal [CL0271] to SCGN/Secretagogin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human pancreas, cerebellum and duodenum tissues. WB: Human brain tissue lysate.
This product was previously labelled as SCGN
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab244219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.|
Tissue specificityExpressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
Sequence similaritiesContains 6 EF-hand domains.
Cellular localizationCytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
- Information by UniProt
- Calbindin like antibody
- CALBL antibody
- DJ501N12.8 antibody
Paraffin-embedded human duodenum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebellum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.
Anti-SCGN/Secretagogin antibody [CL0271] (ab244219) at 1 µg/ml + Human brain tissue lysate
Predicted band size: 32 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244219 has not yet been referenced specifically in any publications.