Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

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Overview

  • Product name

    Anti-SCGN/Secretagogin antibody [CL0271]
    See all SCGN/Secretagogin primary antibodies

  • Description

    Mouse monoclonal [CL0271] to SCGN/Secretagogin

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human SCGN/Secretagogin aa 135-273.
    Sequence:

    RDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENF LLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQ PSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLK


    Database link: O76038

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human pancreas, cerebellum and duodenum tissues. WB: Human brain tissue lysate.

  • General notes

     This product was previously labelled as SCGN

     

Properties

Applications

Our Abpromise guarantee covers the use of ab244219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • Tissue specificity

    Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.

  • Sequence similarities

    Contains 6 EF-hand domains.

  • Cellular localization

    Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
  • Information by UniProt

  • Database links

    • Alternative names

      • Calbindin like antibody
      • CALBL antibody
      • DJ501N12.8 antibody
      • OTTHUMP00000016124 antibody
      • Scgn antibody
      • SECRET antibody
      • Secretagogin antibody
      • Secretagogin EF hand calcium binding protein antibody
      • SEGN antibody
      • SEGN_HUMAN antibody
      • Setagin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human duodenum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human pancreas tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human cerebellum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Western blot - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Western blot - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Anti-SCGN/Secretagogin antibody [CL0271] (ab244219) at 1 µg/ml + Human brain tissue lysate

      Predicted band size: 32 kDa

    References

    ab244219 has not yet been referenced specifically in any publications.

    Publishing research using ab244219? Please let us know so that we can cite the reference in this datasheet.

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