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    scgnsecretagogin-antibody-cl0271-ab244219.pdf

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Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
  • Western blot - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

Key features and details

  • Mouse monoclonal [CL0271] to SCGN/Secretagogin
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-SCGN/Secretagogin antibody [CL0271]
    See all SCGN/Secretagogin primary antibodies
  • Description

    Mouse monoclonal [CL0271] to SCGN/Secretagogin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SCGN/Secretagogin aa 135-273.
    Sequence:

    RDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENF LLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQ PSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLK


    Database link: O76038
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human pancreas, cerebellum and duodenum tissues. WB: Human brain tissue lysate.
  • General notes

     This product was previously labelled as SCGN

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL0271
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Recombinant Protein

    • Recombinant Human SCGN/Secretagogin protein (ab99417)
  • Related Products

    • Recombinant Human SCGN/Secretagogin protein (ab99417)

Applications

Our Abpromise guarantee covers the use of ab244219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • Tissue specificity

    Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
  • Sequence similarities

    Contains 6 EF-hand domains.
  • Cellular localization

    Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
  • Target information above from: UniProt accession O76038 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10590 Human
    • Omim: 609202 Human
    • SwissProt: O76038 Human
    • Unigene: 116428 Human
    • Alternative names

      • Calbindin like antibody
      • CALBL antibody
      • DJ501N12.8 antibody
      • OTTHUMP00000016124 antibody
      • Scgn antibody
      • SECRET antibody
      • Secretagogin antibody
      • Secretagogin EF hand calcium binding protein antibody
      • SEGN antibody
      • SEGN_HUMAN antibody
      • Setagin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human duodenum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human pancreas tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)

      Paraffin-embedded human cerebellum tissue stained for SCGN/Secretagogin using ab244219 at 1/2500 dilution in immunohistochemical analysis.

    • Western blot - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Western blot - Anti-SCGN/Secretagogin antibody [CL0271] (ab244219)
      Anti-SCGN/Secretagogin antibody [CL0271] (ab244219) at 1 µg/ml + Human brain tissue lysate

      Predicted band size: 32 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab244219? Please let us know so that we can cite the reference in this datasheet.

    ab244219 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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