Recombinant
RabMAb

Recombinant Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)

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Overview

  • Product name

    Anti-SCGN/Secretagogin antibody [EPR7476]
    See all SCGN/Secretagogin primary antibodies

  • Description

    Rabbit monoclonal [EPR7476] to SCGN/Secretagogin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human SCGN/Secretagogin (N terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain, fetal midbrain and C6 lysates; Human gastric neuroendocrine carcinoma and pancreas carcinoma tissues

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as SCGN

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Tissue specificity

      Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.

    • Sequence similarities

      Contains 6 EF-hand domains.

    • Cellular localization

      Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
    • Information by UniProt

    • Database links

      • Alternative names

        • Calbindin like antibody
        • CALBL antibody
        • DJ501N12.8 antibody
        • OTTHUMP00000016124 antibody
        • Scgn antibody
        • SECRET antibody
        • Secretagogin antibody
        • Secretagogin EF hand calcium binding protein antibody
        • SEGN antibody
        • SEGN_HUMAN antibody
        • Setagin antibody
        see all

      Images

      • Western blot - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        Western blot - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        All lanes : Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493) at 1/1000 dilution

        Lane 1 : Fetal brain lysate
        Lane 2 : Fetal midbrain lysate
        Lane 3 : C6 lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 32 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)

        Immunohistochemical analysis of paraffin-embedded Human gastric neuroendocrine carcinoma tissue labelling SCGN/Secretagogin with ab138493 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)

        Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labelling SCGN/Secretagogin with ab138493 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Other - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        Other - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab138493 has not yet been referenced specifically in any publications.

      Publishing research using ab138493? Please let us know so that we can cite the reference in this datasheet.

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