Recombinant Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
![Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151472-anti-scgn-antibody-epr7477-western-blot.jpg "Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7477] to SCGN/Secretagogin
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SCGN/Secretagogin antibody [EPR7477]
See all SCGN/Secretagogin primary antibodies -
Description
Rabbit monoclonal [EPR7477] to SCGN/Secretagogin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SCGN/Secretagogin aa 200-300. The exact sequence is proprietary.
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Positive control
- Fetal brain, Human hypothalamus and fetal midbrain lysates; Human gastric neuroendocrine carcinoma and Human pancreas tissues
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General notes
This product was previously labelled as SCGN
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 6.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7477 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. -
Sequence similarities
Contains 6 EF-hand domains. -
Cellular localization
Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage. - Information by UniProt
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Database links
- Entrez Gene: 10590 Human
- Entrez Gene: 214189 Mouse
- Entrez Gene: 306942 Rat
- Omim: 609202 Human
- SwissProt: O76038 Human
- SwissProt: Q91WD9 Mouse
- SwissProt: Q6R556 Rat
- Unigene: 116428 Human
see all -
Alternative names
- Calbindin like antibody
- CALBL antibody
- DJ501N12.8 antibody
see all
Images
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Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)All lanes : Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human hypothalamus lysate
Lane 3 : Fetal midbrain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151471-anti-scgn-antibody-epr7477-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)Immunohistochemical analysis of paraffin-embedded Human gastric neuroendocrine carcinoma tissue labelling SCGN/Secretagogin with ab137017 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151470-anti-scgn-antibody-epr7477-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling SCGN/Secretagogin with ab137017 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151469-anti-scgn-antibody-epr7477-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)Immunohistochemical analysis using ab137017 showing staining in lung small cell undifferentiated carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151468-anti-scgn-antibody-epr7477-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)Immunohistochemical analysis using ab137017 showing staining in Lung carcinoid tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-151467-anti-scgn-antibody-epr7477.jpg)
OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017) -
![Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)](https://www.abcam.com/ps/products/137/ab137017/Images/ab137017-7-benefits-of-recombinant-antibodies.png)
Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137017 has been referenced in 2 publications.
- Yang J et al. A microRNA-24-to-secretagogin regulatory pathway mediates cholesterol-induced inhibition of insulin secretion. Int J Mol Med 44:608-616 (2019). PubMed: 31173188
- Oveland E et al. 1,25-Dihydroxyvitamin-D3 induces brain proteomic changes in cuprizone mice during remyelination involving calcium proteins. Neurochem Int 112:267-277 (2018). IHC-P . PubMed: 28818673