Recombinant
RabMAb

Recombinant Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)

Submit a review Submit a question References (2)

Overview

  • Product name

    Anti-SCGN/Secretagogin antibody [EPR7477]
    See all SCGN/Secretagogin primary antibodies

  • Description

    Rabbit monoclonal [EPR7477] to SCGN/Secretagogin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SCGN/Secretagogin aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, Human hypothalamus and fetal midbrain lysates; Human gastric neuroendocrine carcinoma and Human pancreas tissues

  • General notes

     

     

     This product was previously labelled as SCGN

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Tissue specificity

      Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.

    • Sequence similarities

      Contains 6 EF-hand domains.

    • Cellular localization

      Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Calbindin like antibody
      • CALBL antibody
      • DJ501N12.8 antibody
      • OTTHUMP00000016124 antibody
      • Scgn antibody
      • SECRET antibody
      • Secretagogin antibody
      • Secretagogin EF hand calcium binding protein antibody
      • SEGN antibody
      • SEGN_HUMAN antibody
      • Setagin antibody
      see all

    Images

    • Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      All lanes : Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human hypothalamus lysate
      Lane 3 : Fetal midbrain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)

      Immunohistochemical analysis of paraffin-embedded Human gastric neuroendocrine carcinoma tissue labelling SCGN/Secretagogin with ab137017 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling SCGN/Secretagogin with ab137017 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)

      Immunohistochemical analysis using ab137017 showing staining in lung small cell undifferentiated carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)

      Immunohistochemical analysis using ab137017 showing staining in Lung carcinoid tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Other - Anti-SCGN/Secretagogin antibody [EPR7477] (ab137017)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Yang J  et al. A microRNA-24-to-secretagogin regulatory pathway mediates cholesterol-induced inhibition of insulin secretion. Int J Mol Med 44:608-616 (2019). Read more (PubMed: 31173188) »
    • Oveland E  et al. 1,25-Dihydroxyvitamin-D3 induces brain proteomic changes in cuprizone mice during remyelination involving calcium proteins. Neurochem Int 112:267-277 (2018). IHC-P . Read more (PubMed: 28818673) »
    See all 2 Publications for this product

    Publishing research using ab137017? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab137017.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com