Overview

  • Product name

    Anti-Schistosoma mansoni IgM Human ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    #1 24 3.7%
    #2 24 11.2%
    #3 24 9.8%
    Inter-assay
    Sample n Mean SD CV%
    #6 19 3.7%
    #7 19 3.3%
    #8 24 4.3%
  • Sample type

    Serum, Plasma
  • Assay type

    Indirect
  • Sensitivity

    = 92.6 %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Anti-Schistosoma mansoni IgM Human ELISA Kit is an in vitro enzyme linked immunosorbent assay (ELISA) (ab211773) for the qualitative determination of IgM antibodies against Schistosoma mansoni in human serum or plasma (citrate or heparin). 


    Microplates are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Schistosoma mansoni -specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Schistosoma mansoni -specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.


     

  • Notes

    Schistosomes belong to the class of distomas (trematodes). They rank among the most frequent pathogens. Estimations originate in more than 200 million affected people. The mature parasites are 6 – 22 mm long. The most important species are Schistosoma mansoni, S. japonicum and S. haematobium. Schistosoma mansoni is common in Africa, South America and Middle East.

    Schistosomiasis (bilharziosis) is (depending on species and location of the parasites) a disease of the intestine, liver and spleen resp. urinary passages. Humans are (re)infected by contact with fresh water which is contaminated by ova containing urine or faeces. If larvae bore into human skin, first a transient skin reaction appears (itch with exanthema or erythema, by repeatedly infection cercarial dermatitis is possible). After 3 - 10 weeks the meanwhile sexually mature worms synthesize cytotoxic and allergic substances which course feverish reaction in humans (Katayama fever). The infected person is mostly harmed by the eggs, which get into organs via blood excreting proteins and glycoproteins. The person reacts under participation of own antibodies and immune complexes with formation of granuloma and granulomatous proliferation in intestine and urinary bladder mucosa. Not excreted eggs die after 3 weeks and will be dissolved or calcified. The affected tissue gets fibrous. In final stage bilharziosis will cause death.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab211773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab211773 has not yet been referenced specifically in any publications.

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