Product nameSchneider L2 nuclear extract lysate
See all Schneider L2 lysates
General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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BackgroundThis permanent line is derived from a primary culture of late stage (L2) (20-24 hours old) Drosophila melanogaster embryos (Schneider, 1972) prepared in Scheiders insect medium. It is a positive control for the transcription factor GAGA ab11962.
ab14892 has not yet been referenced specifically in any publications.