Product nameSchneider L2 whole cell lysate
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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BackgroundThis permanent line is derived from a primary culture of late stage (L2) (20-24 hours old) Drosophila melanogaster embryos (Schneider, 1972) prepared in Scheiders insect medium. It is a positive control for the transcription factor GAGA ab11962.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14893 has not yet been referenced specifically in any publications.