Schneider L2 whole cell lysate (ab14893)

Submit a review Q&A (3)

Overview

  • Product name

    Schneider L2 whole cell lysate

  • General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). 

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • Background

    This permanent line is derived from a primary culture of late stage (L2) (20-24 hours old) Drosophila melanogaster embryos (Schneider, 1972) prepared in Scheiders insect medium. It is a positive control for the transcription factor GAGA ab11962.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14893? Please let us know so that we can cite the reference in this datasheet.

ab14893 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I was wondering if your S2 whole cell lysate was active for invitro RNAi activity?

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Answer

Thank you for your enquiry. I am afraid we have not tested this yet so can't provide further information.

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Question

I have an other question : if I had to dilute this product, in wich buffer could I do this. Are the storage conditions different for the diluted aliquots. Thanks.

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Answer

Thank you for your enquiry. The Drosophila SL2 lysate is supplied in 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9. I would advise diluting the lysate in the same buffer and storing in aliquots at -20oC or -80oC. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Question

Hi, I have a couple of question about this product : - which is the quantity of droso cells necessary to obtain the 100 microgram lysate (approximately)? - what's the characteristic of these drosophila cells?, are they different from D.mel S2 (embryonic cells of Drosophila melanogaster)? Thanks

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Answer

Thank you for your enquiry. May I please apologise for the delay to your question enquiring about the number of cells required to derive 100ug of lysate. The cell lysates were generated in large batches and then aliquoted, 100 ug per vial. Our supplier would estimate that it would take about 4 million SL2 cells to obtain 100ug of lysate. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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