Key features and details
- Rabbit polyclonal to SCIN
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SCIN antibody
See all SCIN primary antibodies
DescriptionRabbit polyclonal to SCIN
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- WB: Mouse stomach lysate. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notes>95% pure
Our Abpromise guarantee covers the use of ab223055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 80, 66, 53 kDa).|
FunctionCa(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Adseverin antibody
- ADSV_HUMAN antibody
- KIAA1905 antibody
Anti-SCIN antibody (ab223055) at 1/500 dilution + Mouse stomach tissue lysate
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 80, 66, 53 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SCIN (green) using ab223055 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).
ab223055 has not yet been referenced specifically in any publications.