Overview

  • Product name

    Anti-SCIN antibody [EPR16310] - C-terminal
    See all SCIN primary antibodies
  • Description

    Rabbit monoclonal [EPR16310] to SCIN - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SCIN aa 650 to the C-terminus.
    Database link: Q9Y6U3

  • Positive control

    • WB: Human fetal kidney lysate. Mouse and Rat kidneyand spleen lysates. HEK293, C6, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human clear cell carcinoma of kidney and Human pancreas tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adseverin antibody
    • ADSV_HUMAN antibody
    • KIAA1905 antibody
    • Scin antibody
    • Scinderin antibody
    see all

Images

  • All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cells) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 5 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human clear cell carcinoma of kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Jian W  et al. Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells. Oncol Lett 16:3207-3214 (2018). Read more (PubMed: 30127916) »
See 1 Publication for this product

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