Overview

  • Product name

  • Description

    Rabbit polyclonal to Sclerostin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 50964

  • Positive control

    • WB: HeLa cell lysate. Mouse liver, mouse lung, rat kidney and human kidney tissue lysates. IHC-P: Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/10 - 1/50.

Target

  • Function

    Negative regulator of bone growth.
  • Tissue specificity

    Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days.
  • Involvement in disease

    Defects in SOST are the cause of sclerosteosis (SOST) [MIM:269500]; also known as cortical hyperostosis with syndactyly. SOST is an autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients.
    Note=A 52 kb deletion downstream of SOST results in SOST transcription suppression and is a cause of van Buchem disease (VBCH) [MIM:239100]; also known as hyperostosis corticalis generalisata. VBCH is an autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated.
  • Sequence similarities

    Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BEER antibody
    • CDD antibody
    • Cortical hyperostosis with syndactyly antibody
    • Sclerosteosis antibody
    • Sclerostin antibody
    • Sost antibody
    • SOST_HUMAN antibody
    • SOST1 antibody
    • UNQ2976/PRO7455/PRO7476 antibody
    • VBCH antibody
    see all

Images

  • All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 24 kDa

  • All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 24 kDa

  • ab63097 staining Sclerostin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat polyclonal was used as the secondary antibody.

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling sclerostin with ab63097 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.

    See Abreview

  • Anti-Sclerostin antibody (ab63097) at 1/1000 dilution + HeLa cell lysate at 35 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 24 kDa

  • Anti-Sclerostin antibody (ab63097) at 1/500 dilution + Rat bone (tibia) whole tissue lysate at 36 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 55 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)


    Exposure time: 10 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Sclerostin with ab63097. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

References

This product has been referenced in:

  • Zhang X  et al. Distinctive role of ACVR1 in dentin formation: requirement for dentin thickness in molars and prevention of osteodentin formation in incisors of mice. J Mol Histol 50:43-61 (2019). Read more (PubMed: 30519900) »
  • Ferretti M  et al. Interaction among Calcium Diet Content, PTH (1-34) Treatment and Balance of Bone Homeostasis in Rat Model: The Trabecular Bone as Keystone. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30754633) »
See all 19 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (epithelial cells)
Permeabilization
Yes - Tween
Specification
epithelial cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Oct 15 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (BONE)
Permeabilization
No
Specification
BONE
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (cartilage)
Permeabilization
No
Specification
cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 25 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
Yes - 0.025% Triton x-100
Specification
Bone tissue (Femur)
Blocking step
Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 11 2015

Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Rat Tissue lysate - whole (Bone - Tibia)
Specification
Bone - Tibia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Helder Fonseca

Verified customer

Submitted Sep 17 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (SW1353 human chondrosacroma cell line)
Loading amount
40 µg
Specification
SW1353 human chondrosacroma cell line
Treatment
Transfected with full length human SOST
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris Gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 11 2013

Answer

Thank you for contacting Abcam.

The western blot image for ab63097 was provided by the supplying laboratory of this product. The antibody was not one that was produced by Abcam, however we are confident about the data that is shown on our webpages.

If there is anything else I can help you with, please let me know.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Bone and cartilage)
Specification
Bone and cartilage
Fixative
10% neutral-buffered formalin
Antigen retrieval step
None
Permeabilization
No
Blocking step
Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 24 2011

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