Key features and details
- Rabbit polyclonal to SCLY
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SCLY antibody
See all SCLY primary antibodies
DescriptionRabbit polyclonal to SCLY
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human SCLY aa 395-445. The exact sequence is proprietary.
Database link: Q96I15
- WB: HeLa, HEK-293T, Jurkat, TMCK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab240564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 48 kDa.|
|IP||Use at an assay dependent concentration.
Use at 20 μl/mg of lysate.
FunctionCatalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.
Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- hSCL antibody
- Putative selenocysteine lyase antibody
- SCL antibody
All lanes : Anti-SCLY antibody (ab240564) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TMCK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30 seconds
SCLY was immunoprecipitated from 1 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using ab240564 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab240564 at 1/250 dilution.
Lane 1: ab240564 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240564 has not yet been referenced specifically in any publications.