Product nameAnti-SCLY antibody
See all SCLY primary antibodies
DescriptionRabbit polyclonal to SCLY
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human SCLY aa 395-445. The exact sequence is proprietary.
Database link: Q96I15
- WB: HeLa, HEK-293T, Jurkat, TMCK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab240564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 48 kDa.|
|IP||Use at an assay dependent concentration.
Use at 20 μl/mg of lysate.
FunctionCatalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.
Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- hSCL antibody
- Putative selenocysteine lyase antibody
- SCL antibody
All lanes : Anti-SCLY antibody (ab240564) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TMCK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30 seconds
SCLY was immunoprecipitated from 1 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using ab240564 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab240564 at 1/250 dilution.
Lane 1: ab240564 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab240564 has not yet been referenced specifically in any publications.