Key features and details
- Mouse monoclonal [OTI5E6] to SCMH1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-SCMH1 antibody [OTI5E6]
DescriptionMouse monoclonal [OTI5E6] to SCMH1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SCMH1 aa 1-309. (NP_036368) produced in E.coli.
MQSYTPPSNEFKISMKLEAQDPRNTTSTCIATVVGLTGARLRLRLDGSDN KNDFWRLVDSAEIQPIGNCEKNGGMLQPPLGFRLNASSWPMFLLKTLNGA EMAPIRIFHKEPPSPSHNFFKMGMKLEAVDRKNPHFICPATIGEVRGSEV LVTFDGWRGAFDYWCRFDSRDIFPVGWCSLTGDNLQPPGTKVVIPKNPYP ASDVNTEKPSIHSSTKTVLEHQPGQRGRKPGKKRGRTPKTLISHPISAPS KTAEPLKFPKKRGPKPGSKRKPRTLLNPPPASPTTSTPEPDTSTVPQDAA TIPSSAMQA
Database link: Q96GD3
- WB: HEK-293T transfected with pCMV6-ENTRY SCMH1, whole cell lysate. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, pancreatic carcinoma, prostate carcinoma and bladder tissue.
Clone OTI5E6 (formerly 5E6).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by Tris-EDTA, pH 8.0
|WB||1/2000. Predicted molecular weight: 73 kDa.|
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Tissue specificityStrongly expressed in heart, muscle and pancreas. Weakly expressed in brain, placenta, lung, liver and kidney.
Sequence similaritiesBelongs to the SCM family.
Contains 2 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- Polycomb protein SCMH1 antibody
- SCMH 1 antibody
- SCMH1 antibody
All lanes : Anti-SCMH1 antibody [OTI5E6] (ab236411) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY SCMH1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 73 kDa
Paraffin-embedded human breast adenocarcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human colon adenocarcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic carcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate carcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236411 has not yet been referenced specifically in any publications.