Overview

  • Product name

    Anti-SCML2 antibody [SCMAD14A]
  • Description

    Mouse monoclonal [SCMAD14A] to SCML2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, C terminal. (Human)

  • Positive control

    • HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    SCMAD14A
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
  • Tissue specificity

    Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
  • Sequence similarities

    Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • SCML2 antibody
    • SCML2_HUMAN antibody
    • sex comb on midleg like 2 (Drosophila) antibody
    • Sex comb on midleg like 2 antibody
    • Sex comb on midleg like protein 2 antibody
    • Sex comb on midleg, Drosophila, homolog-like 2 antibody
    • Sex comb on midleg-like protein 2 antibody
    see all

Images

  • Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 77.3 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.

References

This product has been referenced in:

  • Galoian K  et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol 47:465-72 (2015). Read more (PubMed: 26094604) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab51506.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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