Key features and details
- Mouse monoclonal [SCMAD14A] to SCML2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-SCML2 antibody [SCMAD14A]
DescriptionMouse monoclonal [SCMAD14A] to SCML2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment, C terminal. (Human)
- HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab51506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).|
FunctionPutative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
Tissue specificityHighly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
Sequence similaritiesBelongs to the SCM family.
Contains 2 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- SCML2 antibody
- SCML2_HUMAN antibody
- sex comb on midleg like 2 (Drosophila) antibody
Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 77.3 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.
ab51506 has been referenced in 1 publication.
- Galoian K et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol 47:465-72 (2015). PubMed: 26094604