Anti-SCML4 antibody (ab154011)
Key features and details
- Rabbit polyclonal to SCML4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SCML4 antibody -
Description
Rabbit polyclonal to SCML4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-237 of Human SCML4 (UniProt ID: Q8N228).
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Positive control
- SK N SH whole cell lysate; BT483 xenograft tissue; C2C12 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
SCML4 is a putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 256380 Human
- Entrez Gene: 268297 Mouse
- SwissProt: Q8N228 Human
- SwissProt: Q80VG1 Mouse
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Alternative names
- dJ47M23.1 antibody
- FLJ36252 antibody
- Sex comb on midleg like 4 (Drosophila) antibody
see all
Images
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Anti-SCML4 antibody (ab154011) at 1/1000 dilution + SK-N-SH whole cell lysate at 30 µg
Predicted band size: 45 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded BT483 xenograft tissue labeling SCML4 with ab154011 at 1/750 dilution.
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Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue, labeling SCML4 with ab154011 at 1/750 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154011 has not yet been referenced specifically in any publications.