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    scn1a-antibody-ab24820.pdf

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Neuroscience Neurotransmission Receptors / Channels Sodium Channels
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Anti-Scn1a antibody (ab24820)

  • Datasheet
  • SDS
Reviews (7)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab24820)
  • Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (ab24820)

Key features and details

  • Rabbit polyclonal to Scn1a
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Scn1a antibody
    See all Scn1a primary antibodies
  • Description

    Rabbit polyclonal to Scn1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Zebrafish
  • Immunogen

    Synthetic peptide within Human Scn1a aa 1450-1550. The exact sequence is proprietary.
    Database link: P35498

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab with sodium azide is stable for 24 months when stored at 2-8 °C.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P (1)
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • Involvement in disease

    Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
    Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus.
    Defects in SCN1A are a cause of intractable childhood epilepsy with generalized tonic-clonic seizures (ICEGTC) [MIM:607208]. ICEGTC is a disorder characterized by generalized tonic-clonic seizures beginning usually in infancy and induced by fever. Seizures are associated with subsequent mental decline, as well as ataxia or hypotonia. ICEGTC is similar to SMEI, except for the absence of myoclonic seizures.
    Defects in SCN1A are the cause of migraine familial hemiplegic type 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia, and phonophobia. Age at onset ranges from 6 to 15 years. FHM is occasionally associated with other neurologic symptoms such as cerebellar ataxia or epileptic seizures. A unique eye phenotype of elicited repetitive daily blindness has also been reported to be cosegregating with FHM in a single Swiss family.
    Defects in SCN1A are the cause of familial febrile convulsions type 3A (FEB3A) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions are seizures associated with febrile episodes in childhood without any evidence of intracranial infection or defined pathologic or traumatic cause. It is a common condition, affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures (generally defined as generalized onset, single seizures with a duration of less than 30 minutes). Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes, and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following simple febrile seizures is low. Complex febrile seizures are associated with a moderately increased incidence of epilepsy.
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.1/SCN1A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35498 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6323 Human
    • Entrez Gene: 20265 Mouse
    • Entrez Gene: 100009591 Rabbit
    • Entrez Gene: 81574 Rat
    • Entrez Gene: 393101 Zebrafish
    • Omim: 182389 Human
    • SwissProt: P35498 Human
    • SwissProt: P04774 Rat
    • Unigene: 22654 Human
    • Unigene: 365737 Mouse
    • Unigene: 32079 Rat
    see all
  • Alternative names

    • brain sodium channel type I antibody
    • EIEE6 antibody
    • FEB3 antibody
    • FEB3A antibody
    • FHM3 antibody
    • GEFS+2 antibody
    • GEFSP2 antibody
    • HBSC I antibody
    • HBSCI antibody
    • NAC1 antibody
    • Nav 1.1 antibody
    • RBI antibody
    • SCN1 antibody
    • Scn1a antibody
    • SCN1A_HUMAN antibody
    • SMEI antibody
    • Sodium channel protein brain I alpha subunit antibody
    • Sodium channel protein brain I subunit alpha antibody
    • Sodium channel protein type 1 subunit alpha antibody
    • Sodium channel protein type I subunit alpha antibody
    • Sodium channel voltage gated type 1 alpha subunit antibody
    • Sodium channel voltage gated type I alpha polypeptide antibody
    • Voltage-gated sodium channel subunit alpha Nav1.1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab24820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab24820)
    Staining of human cerebellum with anti-sodium channel antibody. The tissue was boiled in 10mM citrate buffer, pH 6.0 for 10 mins followed by cooling at room temparture for 20 mins. The working dilution for the antibody is 1:200 for 30 min at room temparture.
  • Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (ab24820)
    Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (ab24820)
    ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab24820? Please let us know so that we can cite the reference in this datasheet.

ab24820 has been referenced in 5 publications.

  • Wang Z  et al. Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats. Neuroscience 418:231-243 (2019). PubMed: 31473280
  • Hamilton KA  et al. Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus. PLoS One 13:e0196223 (2018). PubMed: 29715265
  • Chen YH  et al. MDH2 is an RNA binding protein involved in downregulation of sodium channel Scn1a expression under seizure condition. Biochim Biophys Acta 1863:1492-1499 (2017). WB . PubMed: 28433711
  • Zhang Y  et al. Pharmacological characterization of an antisense knockdown zebrafish model of Dravet syndrome: inhibition of epileptic seizures by the serotonin agonist fenfluramine. PLoS One 10:e0125898 (2015). PubMed: 25965391
  • Biet M  et al. Prolongation of action potential duration and QT interval during epilepsy linked to increased contribution of neuronal sodium channels to cardiac late Na+ current: potential mechanism for sudden death in epilepsy. Circ Arrhythm Electrophysiol 8:912-20 (2015). PubMed: 26067667

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-Scn1a antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Cell lysate - other (brain zebrafish lysate)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
25 µg
Specification
brain zebrafish lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 19 2019

Question

i like know more about this product. i like use this to inmuno fluorecense in hippocampal neuron culture. you can send me a paper that used this ab for inmune fluorecense,

Read More

Abcam community

Verified customer

Asked on Sep 08 2011

Answer

Thank you for contacting us. We have all the data and Abreviews (submitted by customers) on our online datasheet. Please see: https://www.abcam.com/scn1a-antibody-ab24820.html The ICC/IF information is as follows: ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Unfortunately, we currently are not aware of a paper or publication for this antibody. This might be due to the fact that the catalog number is not mentioned and we do not know if a paper refers to this antibody ab24820 or another Scn1a antibody. However, we do guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 08 2011

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Scn1a antibody

Inconclusive
Abreviews
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Dorsal Root Ganglion)
Specification
Dorsal Root Ganglion
Fixative
Paraformaldehyde
Permeabilization
No
Read More

Dr. Karine Thibault

Verified customer

Submitted Nov 09 2010

Western blot abreview for Anti-Scn1a antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount
10 µg
Specification
Whole brain homogenate
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mr. Brian Hitt

Verified customer

Submitted May 11 2010

Western blot abreview for Anti-Scn1a antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampal Synaptosome Preparation)
Loading amount
4 µg
Specification
Hippocampal Synaptosome Preparation
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 25 2010

Western blot abreview for Anti-Scn1a antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
10 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Dec 16 2009

Western blot abreview for Anti-Scn1a antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 07 2008

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Scn1a antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Vlady Pavlova

Verified customer

Submitted Dec 11 2007

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