• Product name

    Anti-SCN2A antibody
  • Description

    Rabbit polyclonal to SCN2A
  • Host species

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N terminal of human SCN2A.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab65163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 228 kDa.


  • Function

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • Involvement in disease

    Defects in SCN2A are a cause of generalized epilepsy with febrile seizures plus (GEFS+) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
    Defects in SCN2A are the cause of benign familial infantile convulsions type 3 (BFIC3) [MIM:607745]. BFIC3 is an autosomal dominant disorder in which afebrile seizures occur in clusters during the first year of life, without neurologic sequelae.
    Defects in SCN2A are the cause of epileptic encephalopathy early infantile type 11 (EIEE11) [MIM:613721]. EIEE11 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities.
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.2/SCN2A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational

    May be ubiquitinated by NEDD4L; which would promote its endocytosis.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • HBSC II antibody
    • NAC2 antibody
    • Scn2a antibody
    • SCN2A_HUMAN antibody
    • SCN2A1 antibody
    • SCN2A2 antibody
    • Sodium channel protein brain II subunit alpha antibody
    • Sodium channel protein type 2 subunit alpha antibody
    • Sodium channel protein type II subunit alpha antibody
    • Sodium channel protein, brain II subunit alpha antibody
    • Voltage gated sodium channel subunit alpha Nav1.2 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.2 antibody
    see all


  • ICC/IF image of ab65163 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65163, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab65163 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your patience.

I had the lab compare the sequence to the immunogen and unfortunately there is homology between your sequence and the immunogen of ab65163.

I am sorry that this information could not be more helpful. Please let me know if you have any other questions.

Read More


Thank you for your inquiry.

We source this product from an outside laboratory and they consider the exact sequence of the immunogen to be proprietary. If there is a particular region of a.a. you are interested in, please let me know and I can ask them to run a BLAST comparison.

I am sorry that this information could not be more helpful. Please contact us with any other questions.

Read More
Western blot
Mouse Tissue lysate - other (Hippocampal synaptosome preparation)
Loading amount
2 µg
Hippocampal synaptosome preparation
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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