Overview

  • Product name

  • Description

    Rabbit polyclonal to SCN3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SCN3A aa 80-130.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 226 kDa.

Target

  • Function

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.3/SCN3A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    May be ubiquitinated by NEDD4L; which would promote its endocytosis.
    Phosphorylation at Ser-1501 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • brain III voltage-gated sodium channel antibody
    • NAC3 antibody
    • Nav1.3 antibody
    • SCN3A antibody
    • SCN3A_HUMAN antibody
    • Sodium channel protein brain III subunit alpha antibody
    • Sodium channel protein type 3 subunit alpha antibody
    • Sodium channel protein type III subunit alpha antibody
    • Sodium channel protein, brain III subunit alpha antibody
    • sodium channel, brain type III, alpha subunit antibody
    • sodium channel, neuronal type III, alpha subunit antibody
    • sodium channel, voltage-gated, type III, alpha polypeptide antibody
    • sodium channel, voltage-gated, type III, alpha subunit antibody
    • Voltage gated sodium channel subtype III antibody
    • Voltage gated sodium channel subunit alpha Nav1.3 antibody
    • Voltage-gated sodium channel subtype III antibody
    • Voltage-gated sodium channel subunit alpha Nav1.3 antibody
    see all

Images

  • ICC/IF image of ab65164 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65164, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Wang Z  et al. Re-expression of voltage-gated sodium channel subtype Nav1.3 in the substantia nigra after dopamine depletion. Neurosci Lett 687:146-152 (2018). Read more (PubMed: 30267849) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab65164.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up