Anti-SCNN1B antibody (ab172276)
Key features and details
- Mouse polyclonal to SCNN1B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCNN1B antibody
See all SCNN1B primary antibodies -
Description
Mouse polyclonal to SCNN1B -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit -
Immunogen
Recombinant full length protein within Human SCNN1B aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: AAH36352.2 -
Positive control
- Recombinant Human SCNN1B protein (ab132248) can be used as a positive control in WB. SCNN1B transfected 293T cell line lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. |
Target
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Function
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. -
Involvement in disease
Pseudohypoaldosteronism 1, autosomal recessive
Liddle syndrome
Bronchiectasis with or without elevated sweat chloride 1 -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Apical cell membrane. Apical membrane of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 6338 Human
- Omim: 600760 Human
- SwissProt: P51168 Human
- SwissProt: O97742 Rabbit
- Unigene: 414614 Human
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Alternative names
- Amiloride sensitive sodium channel subunit beta antibody
- Amiloride-sensitive sodium channel subunit beta antibody
- Beta NaCH antibody
see all
Images
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All lanes : Anti-SCNN1B antibody (ab172276) at 1 µg/ml
Lane 1 : SCNN1B transfected 293T cell line lysate
Lane 2 : 293T cell line lysate (non-transfected)
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Datasheets and documents
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Datasheet download
References (0)
ab172276 has not yet been referenced specifically in any publications.