Key features and details
- Rabbit polyclonal to SCNN1B (phospho T615)
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCNN1B (phospho T615) antibody
See all SCNN1B primary antibodies
DescriptionRabbit polyclonal to SCNN1B (phospho T615)
SpecificityDetects endogenous levels of SCNN1B only when phosphorylated at threonine 615
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).
- Human brain tissue; extracts from HeLa cells; COS7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 73 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionSodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Involvement in diseasePseudohypoaldosteronism 1, autosomal recessive
Bronchiectasis with or without elevated sweat chloride 1
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationApical cell membrane. Apical membrane of epithelial cells.
- Information by UniProt
- Amiloride sensitive sodium channel subunit beta antibody
- Amiloride-sensitive sodium channel subunit beta antibody
- Beta NaCH antibody
All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.
Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.
ab79172 has been referenced in 1 publication.
- Shimko MJ et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . PubMed: 25347857