Overview

  • Product name

    Anti-SCNN1B (phospho T615) antibody
    See all SCNN1B primary antibodies
  • Description

    Rabbit polyclonal to SCNN1B (phospho T615)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of SCNN1B only when phosphorylated at threonine 615
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).

  • Positive control

    • Human brain tissue; extracts from HeLa cells; COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab79172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • Involvement in disease

    Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1
  • Sequence similarities

    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.
  • Cellular localization

    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

Images

  • All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.

References

This product has been referenced in:

  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . Read more (PubMed: 25347857) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

I have contacted the lab and they have told me that the immunogen sequence for ab79172 is the sequence between *******of the human sequence. If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for contacting Abcam. I have heard back from the lab and they have told me that we currently do not have enough of the immunizing peptide used to generate ab79172 to sell. I am sorry that I could not provide you with the immunizing peptide, but it if there is anything else I can help you with, please let me know.

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