• Product name

  • Description

    Rabbit polyclonal to SCNN1G
  • Host species

  • Specificity

    Detects recombinant rat SCNN1G (gamma ENaC).

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis, Non human primates, Amphibian
  • Immunogen

    Synthetic peptide corresponding to Human SCNN1G aa 630-649.


    (Peptide available as ab4990)

  • General notes

     This product was previously labelled as epithelial Sodium Channel gamma




Our Abpromise guarantee covers the use of ab3468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Can be blocked with SCNN1G peptide (ab4990).
IP Use at an assay dependent concentration. This antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma ENaC protein.


  • Function

    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • Involvement in disease

    Defects in SCNN1G are a cause of Liddle syndrome (LIDDS) [MIM:177200]. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.
    Defects in SCNN1G are the cause of bronchiectasis with or without elevated sweat chloride type 3 (BESC3) [MIM:613071]. A debilitating respiratory disease characterized by chronic, abnormal dilatation of the bronchi and other cystic fibrosis-like symptoms in the absence of known causes of bronchiectasis (cystic fibrosis, autoimmune diseases, ciliary dyskinesia, common variable immunodeficiency, foreign body obstruction). Clinical features include sub-normal lung function, sinopulmonary infections, chronic productive cough, excessive sputum production, and elevated sweat chloride in some cases.
  • Sequence similarities

    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.
  • Post-translational

    Phosphorylated on serine and threonine residues.
    Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.
  • Cellular localization

    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amiloride sensitive epithelial sodium channel gamma subunit antibody
    • Amiloride sensitive sodium channel subunit gamma antibody
    • Amiloride-sensitive sodium channel subunit gamma antibody
    • BESC3 antibody
    • ENaC gamma subunit antibody
    • ENaCG antibody
    • ENaCgamma antibody
    • Epithelial Na(+) channel subunit gamma antibody
    • Epithelial Na+ channel subunit gamma antibody
    • Gamma ENaC antibody
    • Gamma NaCH antibody
    • Gamma-ENaC antibody
    • Gamma-NaCH antibody
    • Nonvoltage gated sodium channel 1 subunit gamma antibody
    • Nonvoltage-gated sodium channel 1 subunit gamma antibody
    • PHA 1 antibody
    • PHA1 antibody
    • SCNEG antibody
    • SCNN 1G antibody
    • SCNN1G antibody
    • SCNNG_HUMAN antibody
    • Sodium channel epithelial 1 gamma subunit antibody
    • Sodium channel non voltage gated 1 gamma subunit antibody
    • Sodium channel nonvoltage gated 1 gamma antibody
    see all


  • All lanes : Anti-SCNN1G antibody (ab3468) at 1/2000 dilution

    Lane 1 : H441 whole cell lysate (treated with dexamethasone)
    Lane 2 : H441 whole cell lysate (treated with dexamethasone) with Immunizing peptide

    Lysates/proteins at 50 µg per lane.

    All lanes : HRP conjugated donkey anti-rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 90-95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding)

    Exposure time: 15 seconds

    See Abreview

  • ab3468 staining SCNN1G in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% Donkey serum for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in KPBS-BT2) for 12 hours at 4°C. An undiluted HRP-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview


This product has been referenced in:

  • Hou Y  et al. Upregulation of the WNK4 Signaling Pathway Inhibits Epithelial Sodium Channels of Mouse Tracheal Epithelial Cells After Influenza A Infection. Front Pharmacol 10:12 (2019). Read more (PubMed: 30723408) »
  • Sun X  et al. Activation of the epithelial sodium channel (ENaC) leads to cytokine profile shift to pro-inflammatory in labor. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 30154237) »
See all 19 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A


Vielen Dank für Ihre Anfrage.

Nein, es handelt sich um kein Missverständnis: Ich wollte mit den Wikipedia Eintragen nur verdeutlichen, dass es bei HEK293 Zellen nicht ganz klar ist, um welche Art von Zellen es sich handelt; bzw. ob es möglich ist, dass diese Zellen das Protein von sich aus expremieren.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!

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Es kann gut sein, dass HEK293 Zellen dieses Protein bereits endogen expremieren. Wir selber haben dies allerdings nicht gemessen.

Es gibt allerdings Hinweise, dass dies tatsächlich der Fall sein könnte: Aus Wikipedia "For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell all of which are abundant in kidney. However the fact that the cells originated from cultured kidney cells does not say much about the exact cellular origin of the HEK 293, as embryonic kidney cultures may contain small numbers of almost all cell types of the body, including neural crest cells, neurons and glia. In fact Graham and coworkers more recently provided evidence that HEK 293 cells and several other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus was taken up and transformed a neuronal lineage cell in the original kidney culture." Laut UniGene kann man sowohl in der Niere als auch im Nervengewebe RNA dieses Proteins nachweisen.

Die Höhe der Bande kann aufgrund des Glykosylierungsgrad dieses Proteins erklärt werden.

Ich möchte Vorschlagen, eine Negativkontrolle zu verwenden, die dieses Protein garantiert nicht expremiert, ZB. eine mit siRNA behandelte Zellkultur ,oder eine Gewebeprobe von einem Gewebe, das dieses Protein nicht expremiert (Thymus, Lymphe, Muskel). Eine Zellkultur aus so einem Gewebe (wie Jurkat Zellen) ist auch eine gute Lösung.

Falls Sie immer noch diese ominöse Doppelbande nach Verwendung einer Negativkontrolle sehen, wenden Sie sich bitte sofort an uns.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: heat with pH 6.4
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C

Abcam user community

Verified customer

Submitted May 12 2012

Western blot
Mouse Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
10 µg
1X10^-6 M Vp for 30 min
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C

Abcam user community

Verified customer

Submitted May 12 2012

Western blot
Human Cell lysate - whole cell (bronchial)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 28 2011

Western blot
Human Cell lysate - whole cell (H441)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 30 2007


Thank you for your enquiry and I apologize for the confusing information that is provided for ab2906 and ab3468. I will verify whether these Abs work in WBs. Once I have that information, I will correct the datasheets and e-mail a copy to you. Thank you for your patience and I will get back to you by Mon.

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