Anti-SCOC antibody (ab239072)
Key features and details
- Rabbit polyclonal to SCOC
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCOC antibody
See all SCOC primary antibodies -
Description
Rabbit polyclonal to SCOC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SCOC aa 1-121.
Database link: Q9UIL1 -
Positive control
- ICC/IF: HepG2 cells. IHC-P: Human skeletal muscle and heart tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab239072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/500. | |
| IHC-P | 1/20 - 1/200. |
Target
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Tissue specificity
Mostly expressed in brain, heart and skeletal muscle. -
Sequence similarities
Belongs to the SCOC family. -
Cellular localization
Nucleus. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 60592 Human
- SwissProt: Q9UIL1 Human
- Unigene: 480815 Human
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Alternative names
- HRIHFB2072 antibody
- Scoc antibody
- SCOC_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SCOC antibody (ab239072)
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SCOC using ab239072 at a dilution of 1/266 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCOC antibody (ab239072)Paraffin-embedded human heart tissue stained for SCOC with ab239072 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCOC antibody (ab239072)Paraffin-embedded human skeletal muscle tissue stained for SCOC with ab239072 at a 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab239072 has not yet been referenced specifically in any publications.
