Overview

  • Product name
  • Description
    Rabbit polyclonal to SCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide (Mouse) (C terminal).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/750.
IHC-P 1/750.
ICC/IF 1/100 - 1/750.

Target

  • Function
    Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
  • Tissue specificity
    Testis.
  • Domain
    Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
  • Cellular localization
    Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 8 antibody
    • CT8 antibody
    • HOM TES 14 antibody
    • MGC104417 antibody
    • SCP 1 antibody
    • SCP-1 antibody
    • SCP1 antibody
    • SYCP 1 antibody
    • Sycp1 antibody
    • SYCP1_HUMAN antibody
    • Synaptonemal complex protein 1 antibody
    • Testicular tissue protein Li 191 antibody
    see all

Images

  • SCP1 labeled in mouse pachytene preparation (red), using ab15090. CDK2 staining, near teleomeres, is also present (green).
  • Paraffin embeded IHC image of Murine SCP1 in mouse ovary using ab15090 at 1:750 dilution and secondary at 1:400.

References

This product has been referenced in:
See all 42 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Permeabilization
Yes - Triton
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse testes)
Specification
mouse testes
Fixative
4% PFA
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 07 2013

Answer

Thank you for contacting Abcam.

Under our Abpromise we guarantee our products for 6 months from the date of delivery and so it is no longer covered under this guarantee. I will be more than happy to help you troubleshoot your protocol to see if there are any changes that I can suggest that will help.

Also, if you are concerned that the staining pattern is incorrect, I would recommend speaking to your lab colleague as to why they felt the staining was specific enough to publish with and also why they did not question the specificity of the antibody. Also were you using the exact same protocol as your lab colleague?

Please let me know if there is anything else I can help you with.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatocytes)
Specification
Spermatocytes
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%

Abcam user community

Verified customer

Submitted May 02 2007

Answer

I can confirm that ab15090 is not likely to cross react with human as the immunogen shares only 83% homology with the human protein. Unfortunately ab15087 will work in human but does not appear to work in immunohistochemistry on paraffin-embedded sections. I'm sorry we don't appear to have a product suitable for your needs at the moment,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up