Anti-SCP1 antibody [EP7918] (ab175191)

Overview

  • Product name
    Anti-SCP1 antibody [EP7918]
    See all SCP1 primary antibodies
  • Description
    Rabbit monoclonal [EP7918] to SCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SCP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15431

  • Positive control
    • Human testis tissue; Mouse and Rat testis tissue lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP7918
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
    • Tissue specificity
      Testis.
    • Domain
      Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
    • Cellular localization
      Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 8 antibody
      • CT8 antibody
      • HOM TES 14 antibody
      • MGC104417 antibody
      • SCP 1 antibody
      • SCP-1 antibody
      • SCP1 antibody
      • SYCP 1 antibody
      • Sycp1 antibody
      • SYCP1_HUMAN antibody
      • Synaptonemal complex protein 1 antibody
      • Testicular tissue protein Li 191 antibody
      see all

    Images

    • All lanes : Anti-SCP1 antibody [EP7918] (ab175191) at 1/1000 dilution

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Rat testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 114 kDa

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SCP1 with ab175191 at a 1/100 dilution.

    References

    ab175191 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (testis 7-wks-old male mouse)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Specification
    testis 7-wks-old male mouse
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 1%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 16 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Adult testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Buffer/Enzyme Used: 10 mM Sodium Citrate 0.05% Tween-20
    Permeabilization
    No
    Specification
    Adult testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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