Overview

  • Product name
  • Description
    Rabbit polyclonal to SCP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide (Human) (C terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab15093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/500.
IHC-FoFr 1/500. (see Abreview)

Target

  • Function
    Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
  • Tissue specificity
    Testis-specific.
  • Involvement in disease
    Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
  • Sequence similarities
    Belongs to the XLR/SYCP3 family.
  • Cellular localization
    Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Information by UniProt
  • Database links
  • Alternative names
    • choline phosphotransferase 1 antibody
    • chpt1 antibody
    • COR 1 antibody
    • COR1 antibody
    • MGC71888 antibody
    • RNASCP3 antibody
    • SCP 3 antibody
    • SCP-3 antibody
    • SCP3 antibody
    • SPGF4 antibody
    • Sycp 3 antibody
    • Sycp3 antibody
    • SYCP3_HUMAN antibody
    • Synaptonemal complex protein 3 antibody
    see all

Images

  • SCP3 labeled in mouse pachytene preparation (red), using ab15093. CDK2 staining, near teleomeres, is also present (green).
  • ab15093 staining SCP3 in Mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150081 (1/500) was used as the secondary antibody.

    See Abreview

  • ab15093 staining SCP3 in Human TEFNA testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 5% FCS + 0.1% Triton X-100) for 1 hour at 25°C. Ab6721 (1/1000) was used as the secondary antibody.

    See Abreview

  • Ab15093 staining human normal testis tissue. Staining is localized to nuclear compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab15093 staining SCP3 in Mouse testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at 1/200 dilution

  • ab15093 staining SCP3 (green) in Rat testis (spermatocytes) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI (blue).

    See Abreview

  • ab15093 staining SCP3 in pig spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton x100 and blocking with 0.15% BSA was performed for 30 minutes at 250C. Samples were incubated with primary antibody (1/100: in PBT: PBS + 0.15% BSA+0.1% Tween 20) for 12 hours at 25°C. An Alexa Fluor®594-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.

    See Abreview

  • ab15093 staining SCP3 in Mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA and blocked with 5% BSA + 0.3% Triton X-100 for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted FITC-conjugated Donkey anti-Rabbit IgG (H+L) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Wang H  et al. Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice. Cell Death Dis 9:76 (2018). Read more (PubMed: 29362488) »
  • Zhao Y  et al. In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells. Stem Cell Reports 10:509-523 (2018). ICC/IF ; Human . Read more (PubMed: 29398481) »
See all 86 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Spermatocytes)
Permeabilization
Yes - Tween-20
Specification
Spermatocytes
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Mar 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Other
Sample
Human Tissue sections (TEFNA human testis)
Specification
TEFNA human testis
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 26 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4% PFA

Dr. Qing Wen

Verified customer

Submitted Apr 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4% PFA

Dr. Qing Wen

Verified customer

Submitted May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 07 2013

Question
Answer

Comme convenu j'ai mis en place l'envoi de 2 unités de ab15093, le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Dorénavant, vos commandes qui seront traitées le vendredi ne partiront que le lundi suivant afin d'éviter que les produits ne restent en transit tout le weekend. Nous avons ajouté une note sur votre compte client mais je vous conseille tout de même d'ajouter, si possible, sur vos futures commandes la note "ne pas envoyer le vendredi".

Veuillez noter que l'anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

Read More

Question

Product code: 15093
Lot number: GR52043-2

Inquiry: No detection in nucleus

Abcam IHC Questionnaire
1) Abcam product code
ab 15093
2) Abcam order reference number or product batch number
GR52043-2, 2-1) storage temperature of antibody
4℃
3) Description of the problem
No detection in nucleus
4) Sample preparation:
Species: mouse testis
Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin
fixed(10% NBF fixed), paraffin embedded sections, cells in culture, other:
Sample preparation
Positive control: mouse testis
Negative control: mouse testis
5) Fixation step: Yes
If yes: Fixative agent and concentration: 10% NBF
Fixation time: 48hrs
Fixation temperature: 4℃
6) Antigen retrieval method
Microwave 20min in Target retrieval solution (Dako) : DPBS= 1 : 10.
7) Permeabilization method: 0.1% triton X-100 in 15min
Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution
buffers? 1x DPBS
Permeabilizing agent and concentration:
0.1%
8) Blocking agent (eg BSA, serum…): protein block x0909(DAKO)
Concentration: 1X protein block
Blocking time: 1hrs
Blocking temperature: Room temperature
9) Endogenous peroxidases blocked?
㈜다윈바이오 (T. 1588-9741, F. 031-790-4360)
Endogenous biotins blocked?
10) Primary antibody (If more than one was used, describe in “additional notes”) : ab 15093
Concentration or dilution 1:100
Diluent buffer: 1X DPBS
Incubation time: 4℃ Over Night
11) Secondary antibody: (A-21429) Alexa Fluor® 555 goat anti–rabbit IgG antibody
Species: mouse
Isotype: IgG
Reacts against: Rabbit
Concentration or dilution: 1:200
Diluent buffer: dPBS
Incubation time: 1hr
Fluorochrome or enzyme conjugate: Alexa Fluor® 555
12) Washing after primary and secondary antibodies:
Buffer: dPBS
Number of washes: 3 times
13) Detection method
fluorescence
14) How many times have you run this staining? 2 times
Do you obtain the same results every time? Yes
Have you run a "No Primary" control? No
What steps have you altered to try and optimize the use of this antibody?
Yes (mouse germ cell)

Read More
Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably recieved a bad vial.

I apologise for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation.

In addition, I can suggest you may like to consider the following suggestions as a check for future experiments:

1. I can recommend to try different times for antigen retrieval, and different antigen retreival methods? This may require some further optimization.

2. Is the secondary antibody working well with other primary antibodies?


Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human fetal ovary)
Specification
human fetal ovary
Fixative
neutral buffered formalin
Permeabilization
No
Blocking step
5% BSA / 20% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Testis (Spermatocytes))
Specification
Testis (Spermatocytes)
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 26 2010

1-10 of 11 Abreviews or Q&A

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