Anti-SCP3 antibody (ab15093)
Key features and details
- Rabbit polyclonal to SCP3
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SCP3 antibody
See all SCP3 primary antibodies -
Description
Rabbit polyclonal to SCP3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide (Human) (C terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
1/100 - 1/500.
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Notes |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. -
Tissue specificity
Testis-specific. -
Involvement in disease
Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. -
Sequence similarities
Belongs to the XLR/SYCP3 family. -
Cellular localization
Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. - Information by UniProt
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Database links
- Entrez Gene: 50511 Human
- Entrez Gene: 20962 Mouse
- Omim: 604759 Human
- SwissProt: Q8IZU3 Human
- SwissProt: P70281 Mouse
- Unigene: 506504 Human
- Unigene: 297977 Mouse
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Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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SCP3 labeled in mouse pachytene preparation (red), using ab15093. CDK2 staining, near teleomeres, is also present (green).
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ab15093 staining SCP3 in pig spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton x100 and blocking with 0.15% BSA was performed for 30 minutes at 250C. Samples were incubated with primary antibody (1/100: in PBT: PBS + 0.15% BSA+0.1% Tween 20) for 12 hours at 25°C. An Alexa Fluor®594-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (188)
ab15093 has been referenced in 188 publications.
- Song Y et al. Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis. Life Sci Alliance 6:N/A (2023). PubMed: 36414375
- Sanchez-Donoso I et al. Massive genome inversion drives coexistence of divergent morphs in common quails. Curr Biol 32:462-469.e6 (2022). PubMed: 34847353
- Wu H et al. A homozygous loss-of-function mutation in FBXO43 causes human non-obstructive azoospermia. Clin Genet 101:55-64 (2022). PubMed: 34595750
- Gill ME et al. Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells. Cytometry A 101:529-536 (2022). PubMed: 35128792
- Marín-Gual L et al. Strategies for meiotic sex chromosome dynamics and telomeric elongation in Marsupials. PLoS Genet 18:e1010040 (2022). PubMed: 35130272