Key features and details
- Rabbit polyclonal to SCP3
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-SCP3 antibody
See all SCP3 primary antibodies
DescriptionRabbit polyclonal to SCP3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Mouse testis whole tissue lysate. IHC-P: Mouse testis tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 28 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
Sequence similaritiesBelongs to the XLR/SYCP3 family.
Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
- Information by UniProt
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
All lanes : Anti-SCP3 antibody (ab235254) at 5 µg/ml
Lane 1 : Mouse testis whole tissue lysate
Lane 2 : Human testis whole tissue lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Rabbit IgG at 1/70000 dilution
Predicted band size: 28 kDa
Exposure time: 30 seconds
Blocking buffer: 5% BLOTTO.
Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).
Counterstained with hematoxylin.
Negative control: Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).
Counterstained with hematoxylin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235254 has not yet been referenced specifically in any publications.