Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
- Datasheet
- Specific References (40)
- Protocols
Overview
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Product nameAnti-SCP3 antibody [Cor 10G11/7]
See all SCP3 primary antibodies -
DescriptionMouse monoclonal [Cor 10G11/7] to SCP3
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Host speciesMouse
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SpecificityThis antibody is an excellent tool for the visualisation of chromosomes in meiotic spreads.
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Tested applicationsSuitable for: IHC-P, ELISA, ICC/IF, IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse, Hamster, Zebrafish
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Immunogen
Hamster COR1/SCP3 protein (expressed as 6x His fusion in bacteria and purified on a Talon under denaturing conditions).
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Positive control
- WB: Testis (Mouse) Tissue Lysate IHC-P: FFPE mouse testis tissue sections.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone numberCor 10G11/7
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab97672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
Target
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FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
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Tissue specificityTestis-specific.
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Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
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Sequence similaritiesBelongs to the XLR/SYCP3 family.
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Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
- Information by UniProt
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Database links
- Entrez Gene: 20962 Mouse
- SwissProt: P70281 Mouse
- Unigene: 297977 Mouse
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Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-257305-ap2696388assy53282edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)IHC image of SCP3 staining in a section of formalin-fixed paraffin-embedded mouse normal testes. using Mouse on Mouse detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab97672 at 1ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/SCP3-Primary-antibodies-ab97672-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Jason Heaney.ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400. -
![Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-257250-ID1041-ab97672-AP2696388Datasheet-Image.jpg)
Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 125 kDa (possible non-specific binding)
Exposure time: 30 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/SCP3-Primary-antibodies-ab97672-19.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-168716-97672-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin Wenab97672 staining SCP3 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Shawki HH et al. MAFB is dispensable for the fetal testis morphogenesis and the maintenance of spermatogenesis in adult mice. PLoS One 13:e0190800 (2018). Read more (PubMed: 29324782) »
- Shi B et al. MORC2B is essential for meiotic progression and fertility. PLoS Genet 14:e1007175 (2018). Read more (PubMed: 29329290) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"