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  1. Link

    scp3-antibody-cor-10g117-ab97672.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

  • Datasheet
Reviews (8) Submit a question References (115)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

Key features and details

  • Mouse monoclonal [Cor 10G11/7] to SCP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

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Overview

  • Product name

    Anti-SCP3 antibody [Cor 10G11/7]
    See all SCP3 primary antibodies
  • Description

    Mouse monoclonal [Cor 10G11/7] to SCP3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Hamster, Zebrafish
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and Human testis tissue lysates; IHC-P: Mouse testis tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Cor 10G11/7
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)
    • Alexa Fluor® 647 Anti-SCP3 antibody [Cor 10G11/7] (ab205847)
    • Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (3)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • Function

    Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
  • Tissue specificity

    Testis-specific.
  • Involvement in disease

    Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
  • Sequence similarities

    Belongs to the XLR/SYCP3 family.
  • Cellular localization

    Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Target information above from: UniProt accession Q8IZU3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50511 Human
    • Entrez Gene: 20962 Mouse
    • Entrez Gene: 25561 Rat
    • Omim: 604759 Human
    • SwissProt: Q8IZU3 Human
    • SwissProt: P70281 Mouse
    • SwissProt: Q63520 Rat
    • Unigene: 506504 Human
    • Unigene: 297977 Mouse
    • Unigene: 34889 Rat
    see all
  • Alternative names

    • choline phosphotransferase 1 antibody
    • chpt1 antibody
    • COR 1 antibody
    • COR1 antibody
    • MGC71888 antibody
    • RNASCP3 antibody
    • SCP 3 antibody
    • SCP-3 antibody
    • SCP3 antibody
    • SPGF4 antibody
    • Sycp 3 antibody
    • Sycp3 antibody
    • SYCP3_HUMAN antibody
    • Synaptonemal complex protein 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Localization of MAFB in adult mouse testis.

    Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    IHC image of SCP3 staining in a section of formalin-fixed paraffin-embedded mouse normal testis, using Mouse on Mouse detection kit, ab127055.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab97672 at 1 µg/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10mins at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Additional bands at: 125 kDa (possible non-specific binding)


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.

    IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.

    The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin Wen

    ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (115)

Publishing research using ab97672? Please let us know so that we can cite the reference in this datasheet.

ab97672 has been referenced in 115 publications.

  • Bagheri-Fam S  et al. Heterozygous deletion of Sox9 in mouse mimics the gonadal sex reversal phenotype associated with campomelic dysplasia in humans. Hum Mol Genet 29:3781-3792 (2021). PubMed: 33305798
  • Lopes F  et al. Chemotherapy induced damage to spermatogonial stem cells in prepubertal mouse in vitro impairs long-term spermatogenesis. Toxicol Rep 8:114-123 (2021). PubMed: 33425685
  • Wang X  et al. Cellular fate of intersex differentiation. Cell Death Dis 12:388 (2021). PubMed: 33846307
  • Yin H  et al. HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis. Nucleic Acids Res 49:5106-5123 (2021). PubMed: 33939832
  • Liu Q  et al. Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis. Cell Death Dis 12:478 (2021). PubMed: 33980814
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MR. Bryan Niedenberger

Verified customer

Submitted Sep 16 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Fish Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: immunosaver 
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 12 2016

Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 22 2016

Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Cell (Ovaries)
Permeabilization
Yes - Triton X-100
Specification
Ovaries
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Dr. xiaochuan tang

Verified customer

Submitted Jun 19 2015

Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 [Cor 10G11/7] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (postnatal 6 day testes)
Specification
postnatal 6 day testes
Permeabilization
No
Fixative
4% PFA
Read More

Dr. Qing Wen

Verified customer

Submitted Apr 04 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Fixative
4% PFA
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Qing Wen

Verified customer

Submitted Mar 18 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult mouse testis section)
Specification
adult mouse testis section
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. 煜欽 林

Verified customer

Submitted Sep 24 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-SCP3 antibody [Cor 10G11/7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic day 16.5 female germ cells)
Specification
embryonic day 16.5 female germ cells
Fixative
Formaldehyde/SDS
Permeabilization
Yes - 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Dr. Jason Heaney

Verified customer

Submitted Sep 30 2011

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