Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-343931-AntiSCP3-antibody-Cor-10G117-immunohistochemistry-E185-mouse-testis.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)")
Key features and details
- Mouse monoclonal [Cor 10G11/7] to SCP3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-SCP3 antibody [Cor 10G11/7]
See all SCP3 primary antibodies -
Description
Mouse monoclonal [Cor 10G11/7] to SCP3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Hamster, Zebrafish
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and Human testis tissue lysates; IHC-P: Mouse testis tissue sections.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Cor 10G11/7 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
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| Notes |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
Target
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Function
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. -
Tissue specificity
Testis-specific. -
Involvement in disease
Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. -
Sequence similarities
Belongs to the XLR/SYCP3 family. -
Cellular localization
Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. - Information by UniProt
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Database links
- Entrez Gene: 50511 Human
- Entrez Gene: 20962 Mouse
- Entrez Gene: 25561 Rat
- Omim: 604759 Human
- SwissProt: Q8IZU3 Human
- SwissProt: P70281 Mouse
- SwissProt: Q63520 Rat
- Unigene: 506504 Human
see all -
Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Localization of MAFB in adult mouse testis.
Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-257305-anti-scp3-antibody-cor-10g11-7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)IHC image of SCP3 staining in a section of formalin-fixed paraffin-embedded mouse normal testis, using Mouse on Mouse detection kit, ab127055.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab97672 at 1 µg/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10mins at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-257250-anti-scp3-antibody-cor-10g11-7-western-blot.jpg)
Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 125 kDa (possible non-specific binding)
Exposure time: 30 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-112251-anti-scp3-antibody-cor-10g11-7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.
The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-168716-AntiSCP3-antibody-Cor-10G117-immunohistochemistry-mouse-adult-testis-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin Wenab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Datasheets and documents
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Datasheet download
References (115)
ab97672 has been referenced in 115 publications.
- Bagheri-Fam S et al. Heterozygous deletion of Sox9 in mouse mimics the gonadal sex reversal phenotype associated with campomelic dysplasia in humans. Hum Mol Genet 29:3781-3792 (2021). PubMed: 33305798
- Lopes F et al. Chemotherapy induced damage to spermatogonial stem cells in prepubertal mouse in vitro impairs long-term spermatogenesis. Toxicol Rep 8:114-123 (2021). PubMed: 33425685
- Wang X et al. Cellular fate of intersex differentiation. Cell Death Dis 12:388 (2021). PubMed: 33846307
- Yin H et al. HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis. Nucleic Acids Res 49:5106-5123 (2021). PubMed: 33939832
- Liu Q et al. Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis. Cell Death Dis 12:478 (2021). PubMed: 33980814
