Product nameAnti-SCP3 antibody [Cor 10G11/7]
See all SCP3 primary antibodies
DescriptionMouse monoclonal [Cor 10G11/7] to SCP3
SpecificityThis antibody is an excellent tool for the visualisation of chromosomes in meiotic spreads.
Tested applicationsSuitable for: IHC-P, ELISA, ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Zebrafish
Predicted to work with: Rat, Hamster
Recombinant full length protein (His-tag) corresponding to Hamster SCP3 aa 1 to the C-terminus. Protein was purified on a Talon under denaturing conditions.
Database link: Q60547
- WB: Testis (Mouse) Tissue Lysate IHC-P: FFPE mouse testis tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Clone numberCor 10G11/7
Our Abpromise guarantee covers the use of ab97672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.|
FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
Sequence similaritiesBelongs to the XLR/SYCP3 family.
Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
- Information by UniProt
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
Localization of MAFB in adult mouse testis.
Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.
Compromised MDC1 and γH2AX signal expansion in Mof deleted pachytene spermatocytes.
Panel A. Immunofluorescence with SYCP3 (red)(ab97672 1/100 dilution) and BRCA1 (green) antibodies in control and Mof cKO spermatocytes. Scale bars, 10 μm.
ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween-20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400.
IHC image of SCP3 staining in a section of formalin-fixed paraffin-embedded mouse normal testis, using Mouse on Mouse detection kit, ab127055.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab97672 at 1 µg/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10mins at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 125 kDa (possible non-specific binding)
Exposure time: 30 seconds
IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.
The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.
ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
This product has been referenced in:
- Ehrmann I et al. An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. Elife 8:N/A (2019). Read more (PubMed: 30674417) »
- Wang Y et al. The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelope. Nat Commun 10:564 (2019). Read more (PubMed: 30718482) »