Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Testis)
Permeabilization
Yes - 0.25% Triton X-100 for 5 minutes
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Additional Notes
Used 4% PFA for 30 mins. Cell in 3D, cultured in ~4mg/mL matrigel (phenol-red free).
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the use of ab97672 in ICC, an application we have not tested before.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Verified customer
Submitted Oct 10 2022