Product nameAnti-Scramblase 1 antibody [EPR14249] - N-terminal
See all Scramblase 1 primary antibodies
DescriptionRabbit monoclonal [EPR14249] to Scramblase 1 - N-terminal
Tested applicationsSuitable for: ICC, WB, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Scramblase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O15162
- A431 cells; A431, HeLa, 293 and HepG2 whole cell lysate (ab7900).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
Tissue specificityExpressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
Sequence similaritiesBelongs to the phospholipid scramblase family.
modificationsKnown to be palmitoylated at one, yet undefined, site.
- Information by UniProt
- Ca(2+) dependent phospholipid scramblase 1 antibody
- Ca(2+)-dependent phospholipid scramblase 1 antibody
- Erythrocyte phospholipid scramblase antibody
All lanes : Anti-Scramblase 1 antibody [EPR14249] - N-terminal (ab180518) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Western blot analysis of immunoprecipitation pellet from 293 lysate immunoprecipitated using ab180518 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution.
Flow cytometric analysis of A431 cells (2% paraformaldehye-fixed) labeling Scramblase 1 with ab180518 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG control (green). Goat anti-rabbit IgG (FITC) secondary antibody was used at 1/75 dilution.
ab180518 has not yet been referenced specifically in any publications.