Recombinant
RabMAb

Anti-Scramblase 1 antibody [EPR14249] - N-terminal (ab180518)

Overview

  • Product name
    Anti-Scramblase 1 antibody [EPR14249] - N-terminal
    See all Scramblase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14249] to Scramblase 1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Scramblase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O15162

  • Positive control
    • A431 cells; A431, HeLa, 293 and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
    May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
  • Tissue specificity
    Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
  • Sequence similarities
    Belongs to the phospholipid scramblase family.
  • Post-translational
    modifications
    Known to be palmitoylated at one, yet undefined, site.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ca(2+) dependent phospholipid scramblase 1 antibody
    • Ca(2+)-dependent phospholipid scramblase 1 antibody
    • Erythrocyte phospholipid scramblase antibody
    • MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of antibody
    • MmTRA1a antibody
    • MmTRA1b antibody
    • Nor1 antibody
    • Phospholipid scramblase 1 antibody
    • PL scramblase 1 antibody
    • PLS1_HUMAN antibody
    • PLSCR 1 antibody
    • Plscr1 antibody
    • Scramblase1 antibody
    • Tra1 antibody
    • Tra1a antibody
    • Tra1b antibody
    • Transplantability-associated protein 1 antibody
    • Tras1 antibody
    • Tras2 antibody
    see all

Images

  • All lanes : Anti-Scramblase 1 antibody [EPR14249] - N-terminal (ab180518) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Western blot analysis of immunoprecipitation pellet from 293 lysate immunoprecipitated using ab180518 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution.

  • Flow cytometric analysis of  A431 cells (2% paraformaldehye-fixed) labeling Scramblase 1 with ab180518 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG control (green). Goat anti-rabbit IgG (FITC) secondary antibody was used at 1/75 dilution.

References

ab180518 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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