• Product name
  • Description
    Rabbit polyclonal to SCRIBBLE
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human SCRIBBLE.

  • Positive control
    • This antibody gave a positive signal in Human Liver tissue lysate. This antibody gave a positive signal in the following methanol fixed cell line: Hek293.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab115240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 195 kDa (predicted molecular weight: 175 kDa).


  • Function
    Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
  • Tissue specificity
    Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
  • Sequence similarities
    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 4 PDZ (DHR) domains.
  • Post-translational
    Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
  • Cellular localization
    Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRIB 1 antibody
    • CRIB1 antibody
    • hScrib antibody
    • LAP 4 antibody
    • LAP4 antibody
    • PDZ domain protein scribble antibody
    • Protein LAP4 antibody
    • Protein scribble homolog antibody
    • SCRB 1 antibody
    • SCRB1 antibody
    • SCRIB 1 antibody
    • Scrib antibody
    • SCRIB_HUMAN antibody
    • SCRIB1 antibody
    • Scribble antibody
    • Scribble homolog 1 antibody
    • scribbled homolog (Drosophila) antibody
    • Vartul antibody
    see all


  • Anti-SCRIBBLE antibody (ab115240) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 175 kDa
    Observed band size: 195 kDa (why is the actual band size different from the predicted?)

    Exposure time: 4 minutes
  • ICC/IF image of ab115240 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115240 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab115240 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab115240.
Please use the links above to contact us or submit feedback about this product.


Sign up