Anti-SCRIBBLE antibody (ab154067)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SCRIBBLE antibody
See all SCRIBBLE primary antibodies -
Description
Rabbit polyclonal to SCRIBBLE -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide contain a sequence corresponding to a region within amino acids 1568-1660 of Human SCRIBBLE (UniProt ID: Q14160).
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Positive control
- HepG2, NIH3T3 and JC whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 175 kDa. |
Target
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Function
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. -
Tissue specificity
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). -
Sequence similarities
Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsUbiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. - Information by UniProt
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Database links
- Entrez Gene: 23513 Human
- Entrez Gene: 105782 Mouse
- Omim: 607733 Human
- SwissProt: Q14160 Human
- SwissProt: Q80U72 Mouse
- Unigene: 436329 Human
- Unigene: 25568 Mouse
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Alternative names
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
Images
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Western blot - Anti-SCRIBBLE antibody (ab154067)Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg
Predicted band size: 175 kDa
5% SDS PAGE -
Western blot - Anti-SCRIBBLE antibody (ab154067)All lanes : Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysates
Lane 2 : JC whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 175 kDa
5% SDS PAGE
Protocols
References
This product has been referenced in:
- Verbist KC et al. Metabolic maintenance of cell asymmetry following division in activated T lymphocytes. Nature 532:389-93 (2016). Mouse . Read more (PubMed: 27064903) »
