Overview

  • Product name

  • Description

    Rabbit polyclonal to SCRIBBLE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide contain a sequence corresponding to a region within amino acids 1568-1660 of Human SCRIBBLE (UniProt ID: Q14160).

  • Positive control

    • HepG2, NIH3T3 and JC whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 175 kDa.

Target

  • Function

    Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
  • Tissue specificity

    Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
  • Sequence similarities

    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 4 PDZ (DHR) domains.
  • Post-translational
    modifications

    Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
  • Cellular localization

    Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRIB 1 antibody
    • CRIB1 antibody
    • hScrib antibody
    • LAP 4 antibody
    • LAP4 antibody
    • PDZ domain protein scribble antibody
    • Protein LAP4 antibody
    • Protein scribble homolog antibody
    • SCRB 1 antibody
    • SCRB1 antibody
    • SCRIB 1 antibody
    • Scrib antibody
    • SCRIB_HUMAN antibody
    • SCRIB1 antibody
    • Scribble antibody
    • Scribble homolog 1 antibody
    • scribbled homolog (Drosophila) antibody
    • Vartul antibody
    see all

Images

  • Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg

    Predicted band size: 175 kDa



    5% SDS PAGE
  • All lanes : Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution

    Lane 1 : NIH3T3 whole cell lysates
    Lane 2 : JC whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 175 kDa



    5% SDS PAGE

References

This product has been referenced in:

  • Verbist KC  et al. Metabolic maintenance of cell asymmetry following division in activated T lymphocytes. Nature 532:389-93 (2016). Mouse . Read more (PubMed: 27064903) »
See 1 Publication for this product

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