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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus
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Anti-SCRIBBLE antibody (ab154067)

  • Datasheet
  • SDS
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Western blot - Anti-SCRIBBLE antibody (ab154067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067)
  • Western blot - Anti-SCRIBBLE antibody (ab154067)

Key features and details

  • Rabbit polyclonal to SCRIBBLE
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SCRIBBLE antibody
    See all SCRIBBLE primary antibodies
  • Description

    Rabbit polyclonal to SCRIBBLE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide contain a sequence corresponding to a region within amino acids 1568-1660 of Human SCRIBBLE (UniProt ID: Q14160).

  • Positive control

    • WB: HepG2, NIH3T3 and JC whole cell lysate; IHC-P: Human colon carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Human Papillomavirus

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 175 kDa.
IHC-P
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 175 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Function

    Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
  • Tissue specificity

    Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
  • Sequence similarities

    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 4 PDZ (DHR) domains.
  • Post-translational
    modifications

    Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
  • Cellular localization

    Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
  • Target information above from: UniProt accession Q14160 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23513 Human
    • Entrez Gene: 105782 Mouse
    • Omim: 607733 Human
    • SwissProt: Q14160 Human
    • SwissProt: Q80U72 Mouse
    • Unigene: 436329 Human
    • Unigene: 25568 Mouse
    • Alternative names

      • CRIB 1 antibody
      • CRIB1 antibody
      • hScrib antibody
      • LAP 4 antibody
      • LAP4 antibody
      • PDZ domain protein scribble antibody
      • Protein LAP4 antibody
      • Protein scribble homolog antibody
      • SCRB 1 antibody
      • SCRB1 antibody
      • SCRIB 1 antibody
      • Scrib antibody
      • SCRIB_HUMAN antibody
      • SCRIB1 antibody
      • Scribble antibody
      • Scribble homolog 1 antibody
      • scribbled homolog (Drosophila) antibody
      • Vartul antibody
      see all

    Images

    • Western blot - Anti-SCRIBBLE antibody (ab154067)
      Western blot - Anti-SCRIBBLE antibody (ab154067)
      Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

      Secondary
      HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 175 kDa



      5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067)

      Immunohistochemical analysis of human colon carcinoma tissue staining SCRIBBLE at cytoplasm with ab154067 at 1/250 dilution.

      Antigen Retrieval: Trilogy™ EDTA based buffer, pH 8.0, 15min.

    • Western blot - Anti-SCRIBBLE antibody (ab154067)
      Western blot - Anti-SCRIBBLE antibody (ab154067)
      All lanes : Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
      Lane 2 : JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 175 kDa



      5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab154067? Please let us know so that we can cite the reference in this datasheet.

    ab154067 has been referenced in 1 publication.

    • Verbist KC  et al. Metabolic maintenance of cell asymmetry following division in activated T lymphocytes. Nature 532:389-93 (2016). Mouse . PubMed: 27064903

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