Anti-SCRIBBLE antibody (ab154067)
Key features and details
- Rabbit polyclonal to SCRIBBLE
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SCRIBBLE antibody
See all SCRIBBLE primary antibodies -
Description
Rabbit polyclonal to SCRIBBLE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide contain a sequence corresponding to a region within amino acids 1568-1660 of Human SCRIBBLE (UniProt ID: Q14160).
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Positive control
- WB: HepG2, NIH3T3 and JC whole cell lysate; IHC-P: Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 175 kDa.
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| IHC-P |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 175 kDa. |
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IHC-P
1/100 - 1/1000. |
Target
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Function
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. -
Tissue specificity
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). -
Sequence similarities
Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsUbiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. - Information by UniProt
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Database links
- Entrez Gene: 23513 Human
- Entrez Gene: 105782 Mouse
- Omim: 607733 Human
- SwissProt: Q14160 Human
- SwissProt: Q80U72 Mouse
- Unigene: 436329 Human
- Unigene: 25568 Mouse
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Alternative names
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
Images
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Western blot - Anti-SCRIBBLE antibody (ab154067)Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 175 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067)Immunohistochemical analysis of human colon carcinoma tissue staining SCRIBBLE at cytoplasm with ab154067 at 1/250 dilution.
Antigen Retrieval: Trilogy™ EDTA based buffer, pH 8.0, 15min.
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Western blot - Anti-SCRIBBLE antibody (ab154067)All lanes : Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 175 kDa
5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154067 has been referenced in 1 publication.
- Verbist KC et al. Metabolic maintenance of cell asymmetry following division in activated T lymphocytes. Nature 532:389-93 (2016). Mouse . PubMed: 27064903