Key features and details
- Rabbit polyclonal to SCRIBBLE
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCRIBBLE antibody
See all SCRIBBLE primary antibodies
DescriptionRabbit polyclonal to SCRIBBLE
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fusion protein corresponding to C terminus of human SCRIBBLE (including PDZ3 and 4).
- Diluted human cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab36708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/5000. Predicted molecular weight: 175 kDa.|
FunctionScaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
Tissue specificityExpressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Sequence similaritiesBelongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains.
modificationsUbiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
Cellular localizationCell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
- Information by UniProt
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
ICC/IF image of ab36708 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-SCRIBBLE antibody (ab36708) at 1/5000 dilution
All lanes : Whole cell lysate prepared from prostate cancer cells
Lysates/proteins at 40 µg per lane.
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 175 kDa
Observed band size: 175 kDa
Exposure time: 1 minute
No unspecific bands detected.
ab36708 has been referenced in 1 publication.
- Ihry RJ et al. Genome-Scale CRISPR Screens Identify Human Pluripotency-Specific Genes. Cell Rep 27:616-630.e6 (2019). PubMed: 30970262