Recombinant
RabMAb

Recombinant Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

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Overview

  • Product name

    Anti-SCRIBBLE antibody [EPR4140(2)]
    See all SCRIBBLE primary antibodies

  • Description

    Rabbit monoclonal [EPR4140(2)] to SCRIBBLE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4140(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

    • Tissue specificity

      Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

    • Sequence similarities

      Belongs to the LAP (LRR and PDZ) protein family.
      Contains 16 LRR (leucine-rich) repeats.
      Contains 4 PDZ (DHR) domains.

    • Post-translational
      modifications

      Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.

    • Cellular localization

      Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
    • Information by UniProt

    • Database links

      • Alternative names

        • CRIB 1 antibody
        • CRIB1 antibody
        • hScrib antibody
        • LAP 4 antibody
        • LAP4 antibody
        • PDZ domain protein scribble antibody
        • Protein LAP4 antibody
        • Protein scribble homolog antibody
        • SCRB 1 antibody
        • SCRB1 antibody
        • SCRIB 1 antibody
        • Scrib antibody
        • SCRIB_HUMAN antibody
        • SCRIB1 antibody
        • Scribble antibody
        • Scribble homolog 1 antibody
        • scribbled homolog (Drosophila) antibody
        • Vartul antibody
        see all

      Images

      • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
        Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
        All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 175 kDa

      • Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
        Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
        ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

      References

      ab125080 has not yet been referenced specifically in any publications.

      Publishing research using ab125080? Please let us know so that we can cite the reference in this datasheet.

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