Recombinant Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
![Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-420271-anti-scribble-antibody-epr41402-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SCRIBBLE antibody [EPR4140(2)]
See all SCRIBBLE primary antibodies -
Description
Rabbit monoclonal [EPR4140(2)] to SCRIBBLE -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4140(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa).
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| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa). |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. -
Tissue specificity
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). -
Sequence similarities
Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsUbiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. - Information by UniProt
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Database links
- Entrez Gene: 23513 Human
- Omim: 607733 Human
- SwissProt: Q14160 Human
- Unigene: 436329 Human
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Alternative names
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
Images
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Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCRIB knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 175 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.
ab125080 Anti-SCRIBBLE antibody [EPR4140(2)] was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-139233-anti-scribble-antibody-epr41402-western-blot.jpg)
Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 175 kDa -
![Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-139232-anti-scribble-antibody-epr41402-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
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![Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-3-benefits-of-recombinant-antibodies.png)
Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
Datasheets and documents
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Datasheet download
References (0)
ab125080 has not yet been referenced specifically in any publications.
