Recombinant Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SCRIBBLE antibody [EPR4140(2)]
See all SCRIBBLE primary antibodies -
Description
Rabbit monoclonal [EPR4140(2)] to SCRIBBLE -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4140(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa). | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. -
Tissue specificity
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). -
Sequence similarities
Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsUbiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. - Information by UniProt
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Database links
- Entrez Gene: 23513 Human
- Omim: 607733 Human
- SwissProt: Q14160 Human
- Unigene: 436329 Human
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Alternative names
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
Images
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![Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-139233-anti-scribble-antibody-epr41402-western-blot.jpg)
Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 175 kDa -
![Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](https://www.abcam.com/ps/products/125/ab125080/Images/ab125080-139232-anti-scribble-antibody-epr41402-flow-cytometry.jpg)
Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
Protocols
Datasheets and documents
References (0)
ab125080 has not yet been referenced specifically in any publications.
