Anti-SCUBE3 antibody (ab189955)
Key features and details
- Rabbit polyclonal to SCUBE3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SCUBE3 antibody -
Description
Rabbit polyclonal to SCUBE3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SCUBE3 (internal sequence). The exact sequence is proprietary. 15 amino acid peptide
Database link: Q8IX30 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 109 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 109 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Highly expressed in osteoblasts. Expression is low or absent in non-bone tissues. -
Sequence similarities
Contains 1 CUB domain.
Contains 9 EGF-like domains. -
Post-translational
modificationsN-glycosylated.
May undergo proteolytic cleavage in the C-terminal region. -
Cellular localization
Secreted. Cell surface. - Information by UniProt
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Database links
- Entrez Gene: 222663 Human
- Entrez Gene: 268935 Mouse
- Omim: 614708 Human
- SwissProt: Q8IX30 Human
- SwissProt: Q66PY1 Mouse
- Unigene: 12923 Human
- Unigene: 258491 Mouse
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Alternative names
- CEGF3 antibody
- CUB and EGF containing protein 3 antibody
- CUB and EGF-like domain-containing protein 3 antibody
see all
Images
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Lane 1 : Anti-SCUBE3 antibody (ab189955) at 1 µg/ml
Lane 2 : Anti-SCUBE3 antibody (ab189955) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Predicted band size: 109 kDa -
Immunofluorescence staining of Human kidney tissue, labeling SCUBE3 using ab189955 at 20 ug/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling SCUBE3 using ab189955 at 5 µg/ml
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab189955 has been referenced in 1 publication.
- Huo Q et al. SCUBE3 serves as an independent poor prognostic factor in breast cancer. Cancer Cell Int 21:268 (2021). PubMed: 34006286