Product nameAnti-SCXA antibody
See all SCXA primary antibodies
DescriptionRabbit polyclonal to SCXA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
Synthetic peptide corresponding to Mouse SCXA aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q64124
- This antibody gave a positive signal in the following whole cell lysates: TE671; HT1080; SAOS-2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
FunctionPlays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- Basic helix loop helix transcription factor scleraxis antibody
- Basic helix-loop-helix transcription factor scleraxis antibody
- bHLHa41 antibody
All lanes : Anti-SCXA antibody (ab185940) at 1/250 dilution
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab185940 has not yet been referenced specifically in any publications.