Anti-SCXA antibody (ab185940)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SCXA antibody
See all SCXA primary antibodies -
Description
Rabbit polyclonal to SCXA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Mouse SCXA aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q64124 -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: TE671; HT1080; SAOS-2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab185940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100129885 Human
- Entrez Gene: 642658 Human
- Entrez Gene: 20289 Mouse
- GenBank: GC08P145397 Human
- SwissProt: Q7RTU7 Human
- SwissProt: Q64124 Mouse
- Unigene: 553250 Human
- Unigene: 723088 Human
see all -
Alternative names
- Basic helix loop helix transcription factor scleraxis antibody
- Basic helix-loop-helix transcription factor scleraxis antibody
- bHLHa41 antibody
see all
Images
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Western blot - Anti-SCXA antibody (ab185940)All lanes : Anti-SCXA antibody (ab185940) at 1/250 dilution
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab185940 has not yet been referenced specifically in any publications.
