Overview

  • Product name

  • Description

    Rabbit polyclonal to SCXA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Mouse SCXA aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: Q64124

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: TE671; HT1080; SAOS-2.

Properties

Applications

Our Abpromise guarantee covers the use of ab185940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

Images

  • All lanes : Anti-SCXA antibody (ab185940) at 1/250 dilution

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab185940 has not yet been referenced specifically in any publications.

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