Overview

  • Product name

  • Description

    Rabbit polyclonal to SCXA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-M, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Horse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human SCXA

Properties

Applications

Our Abpromise guarantee covers the use of ab58655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/500. Predicted molecular weight: 22 kDa.Can be blocked with SCXA peptide (ab214116).

IP enrichment could be done before running a reducing gel. The positive control is purified protein or overpressed cell lysate.

ELISA Use at an assay dependent concentration.
IHC-M Use at an assay dependent concentration. PubMed: 21412429
ICC Use at an assay dependent concentration. PubMed: 21412429

Target

Images

  • Immunofluorescence analysis of human Tenocyte staining SCXA with ab58655 at 1/500 dilution. Undiluted Goat anti-Rabbit IgG (H+L) used as a secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Vermeulen S  et al. Identification of topographical architectures supporting the phenotype of rat tenocytes. Acta Biomater 83:277-290 (2019). Read more (PubMed: 30394345) »
  • Li K  et al. High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-?B signaling. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30825206) »
See all 22 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ligament)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
Permeabilization
Yes - 0.05% triton-x100
Specification
Ligament
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary skeletal muscle cells)
Permeabilization
Yes - Triton
Specification
Primary skeletal muscle cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Tina Yuan

Verified customer

Submitted Sep 18 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tendon)
Permeabilization
Yes - 0.05% triton-x100
Specification
tendon
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Goat Tissue sections (Achilles tendon tissue)
Permeabilization
Yes - Heat induced using Citrate buffer (pH 6)
Specification
Achilles tendon tissue
Blocking step
2.5% Natural goat serum, 0.05% Tween20, 0.1% Triton-X100 in PBS. as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Jaculus jaculus Tissue sections (muscle)
Antigen retrieval step
None - Buffer/Enzyme Used: 3%tritonX
Permeabilization
Yes - 3%triton
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Fixative
Formaldehyde

Mai Tran

Verified customer

Submitted Feb 28 2018

Application
Immunocytochemistry
Sample
Equus Cell (Tenocyte)
Permeabilization
Yes - 0.1% TX-100 (10 min @ RT)
Specification
Tenocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Tenocyte)
Permeabilization
Yes - 0.1% TX-100 (10 min @ RT)
Specification
Tenocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Application
Western blot
Sample
Mouse Cell lysate - nuclear (Heart)
Gel Running Conditions
Reduced Non-Denaturing (Native) (15)
Loading amount
50 µg
Treatment
TAC
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells of Rat (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rat (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Jul 15 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Jul 15 2016

1-10 of 20 Abreviews or Q&A

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