Overview

  • Product name
  • Description
    Rabbit polyclonal to SCXA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-M, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human SCXA

Properties

Applications

Our Abpromise guarantee covers the use of ab58655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/500. Predicted molecular weight: 22 kDa.Can be blocked with SCXA peptide (ab214116).

IP enrichment could be done before running a reducing gel. The positive control is purified protein or overpressed cell lysate.

ELISA Use at an assay dependent concentration.
IHC-M Use at an assay dependent concentration. PubMed: 21412429
ICC Use at an assay dependent concentration. PubMed: 21412429

Target

Images

  • Immunofluorescence analysis of human Tenocyte staining SCXA with ab58655 at 1/500 dilution. Undiluted Goat anti-Rabbit IgG (H+L) used as a secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chen S  et al. Interleukin-6 Promotes Proliferation but Inhibits Tenogenic Differentiation via the Janus Kinase/Signal Transducers and Activators of Transcription 3 (JAK/STAT3) Pathway in Tendon-Derived Stem Cells. Med Sci Monit 24:1567-1573 (2018). Read more (PubMed: 29547593) »
  • Veron AD  et al. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera. BMC Vet Res 14:17 (2018). Read more (PubMed: 29343270) »
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Goat Tissue sections (Achilles tendon tissue)
Permeabilization
Yes - Heat induced using Citrate buffer (pH 6)
Specification
Achilles tendon tissue
Blocking step
2.5% Natural goat serum, 0.05% Tween20, 0.1% Triton-X100 in PBS. as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Jaculus jaculus Tissue sections (muscle)
Antigen retrieval step
None - Buffer/Enzyme Used: 3%tritonX
Permeabilization
Yes - 3%triton
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Fixative
Formaldehyde

Mai Tran

Verified customer

Submitted Feb 28 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Equus Cell (Tenocyte)
Permeabilization
Yes - 0.1% TX-100 (10 min @ RT)
Specification
Tenocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Tenocyte)
Permeabilization
Yes - 0.1% TX-100 (10 min @ RT)
Specification
Tenocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Heart)
Gel Running Conditions
Reduced Non-Denaturing (Native) (15)
Loading amount
50 µg
Treatment
TAC
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells of Rat (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rat (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Jul 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Jul 15 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Antoine Veron

Verified customer

Submitted Aug 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - 0.1% Triton 100X
Specification
Olfactory stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Antoine Veron

Verified customer

Submitted Jun 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Sample
Mouse Tissue sections (E11.5 embryos)
Specification
E11.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 06 2014

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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