Key features and details
- Rabbit polyclonal to SCY1 like 3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCY1 like 3 antibody
See all SCY1 like 3 primary antibodies
DescriptionRabbit polyclonal to SCY1 like 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: SCY1 like 3 overexpression HEK-293T lysate. IHC-P: Human pancreas tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 83 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in regulating cell adhesion/migration complexes in migrating cells.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 4 HEAT repeats.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
modificationsMay be myristoylated; myristoylation may target it to Golgi compartment.
Cellular localizationCytoplasm. Golgi apparatus. Cell projection > lamellipodium. Colocalized with EZR/VIL2, actin and CD44 in lamellipodia.
- Information by UniProt
- Ezrin binding protein PACE 1 antibody
- Ezrin-binding protein PACE-1 antibody
- PACE 1 antibody
Paraffin-embedded human pancreas tissue stained for SCY1 like 3 using ab224339 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-SCY1 like 3 antibody (ab224339) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : SCY1 like 3 overexpression HEK-293T (co-expressed with a C terminal myc-DDK tag, ~3.1 kDa) lysate
Predicted band size: 83 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224339 has not yet been referenced specifically in any publications.