Overview

  • Product name

  • Description

    Rabbit polyclonal to SCYL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 758 and 808 of Human SCYL1 (NP_065731.3).

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab95074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
    Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 3 HEAT repeats.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Cellular localization

    Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Coated vesicle associated kinase of 90 kDa antibody
    • Coated vesicle-associated kinase of 90 kDa antibody
    • CVAK90 antibody
    • GKLP antibody
    • HT019 antibody
    • Likely ortholog of mouse N terminal kinase like protein antibody
    • MGC78454 antibody
    • N terminal kinase like antibody
    • N terminal kinase like protein antibody
    • N-terminal kinase-like protein antibody
    • NKTL antibody
    • NTKL antibody
    • NTKL_HUMAN antibody
    • NTKLBP1 antibody
    • P105 antibody
    • SCY L1 antibody
    • SCY1 like 1 antibody
    • SCY1 like 1 protein 1 antibody
    • SCY1-like protein 1 antibody
    • SCYL 1 antibody
    • Scyl1 antibody
    • SCYL1BP1 antibody
    • TAPK antibody
    • TEIF antibody
    • Telomerase regulation associated protein antibody
    • Telomerase regulation-associated protein antibody
    • Telomerase transcriptional element interacting factor antibody
    • Telomerase transcriptional element-interacting factor antibody
    • Teratoma associated tyrosine kinase antibody
    • Teratoma-associated tyrosine kinase antibody
    • TRAP antibody
    see all

Images

  • All lanes : Anti-SCYL1 antibody (ab95074) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 90 kDa


    Exposure time: 10 seconds
  • Detection of SCYL1 by Western Blot of Immunprecipitate.
    ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab95074 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Lawrence Welch

Verified customer

Submitted Jun 20 2019

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