Key features and details
- Rabbit polyclonal to SCYL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SCYL1 antibody
See all SCYL1 primary antibodies
DescriptionRabbit polyclonal to SCYL1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residues 758 and 808 of Human SCYL1 (NP_065731.3).
- HeLa, 293T and NIH3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95074 was affinity purified using an epitope specific to SCYL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 90 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
Cellular localizationNucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
- Information by UniProt
- Coated vesicle associated kinase of 90 kDa antibody
- Coated vesicle-associated kinase of 90 kDa antibody
- CVAK90 antibody
All lanes : Anti-SCYL1 antibody (ab95074) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 90 kDa
Exposure time: 10 seconds
Detection of SCYL1 by Western Blot of Immunprecipitate.
ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab95074 has not yet been referenced specifically in any publications.