Anti-SCYL2 antibody (ab221195)
Key features and details
- Rabbit polyclonal to SCYL2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SCYL2 antibody
See all SCYL2 primary antibodies -
Description
Rabbit polyclonal to SCYL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Hamster -
Immunogen
Recombinant fragment (His-tag) corresponding to Human SCYL2 aa 500 to the C-terminus.
Database link: Q6P3W7 -
Positive control
- HeLa, NIH 3T3 and MCF7 cell lysates; MCF7 cells; 293 cells, treated with control of SCYL2-siRNA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
ab221195 is purified by salting-out and ion-exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 104 kDa.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/1000. Predicted molecular weight: 104 kDa. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. -
Sequence similarities
Belongs to the protein kinase superfamily.
Contains 1 HEAT repeat.
Contains 1 protein kinase domain. -
Post-translational
modificationsAccording to PubMed:15809293, autophosphorylated in presence of poly-L-lysine. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes. - Information by UniProt
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Database links
- Entrez Gene: 55681 Human
- Entrez Gene: 213326 Mouse
- Entrez Gene: 314717 Rat
- Omim: 616365 Human
- SwissProt: Q6P3W7 Human
- SwissProt: Q8CFE4 Mouse
- Unigene: 506481 Human
- Unigene: 27651 Mouse
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Alternative names
- AU015688 antibody
- AW536445 antibody
- BC030932 antibody
see all
Images
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Immunofluorescent analysis of 4% PFA fixed, 0.25 Triton X-100 permeabilized MCF7 cells labeling SCYL2 with ab221195 at 1/1000 dilution, followed by secondary detection with Alexa Fluor 488 conjugated goat anti rabbit IgG at 1/1000 dilution (green). DAPI was used for nuclear counterstaining (blue).
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All lanes : Anti-SCYL2 antibody (ab221195) at 1/1000 dilution
Lane 1 : 293 cell lysate siRNA control
Lane 2 : 293 cell lysate treated with SCYL2 siRNA
Predicted band size: 104 kDa -
All lanes : Anti-SCYL2 antibody (ab221195) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 104 kDa7.5% SDS gel
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221195 has not yet been referenced specifically in any publications.