Overview

  • Product name

  • Description

    Rabbit polyclonal to SCYL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Hamster
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human SCYL2 aa 528-929.
    Sequence:

    ILP FLQQIPSKEP AVLMGILGIY KCTFTHKKLG ITKEQLAGKV LPHLIPLSIE NNLNLNQFNS FISVIKEMLN RLESEHKTKL EQLHIMQEQQ KSLDIGNQMN VSEEMKVTNI GNQQIDKVFN NIGADLLTGS ESENKEDGLQ NKHKRASLTL EEKQKLAKEQ EQAQKLKSQQ PLKPQVHTPV ATVKQTKDLT DTLMDNMSSL TSLSVSTPKS SASSTFTSVP SMGIGMMFST PTDNTKRNLT NGLNANMGFQ TSGFNMPVNT NQNFYSSPST VGVTKMTLGT PPTLPNFNAL SVPPAGAKQT QQRPTDMSAL NNLFGPQKPK VSMNQLSQQK PNQWLNQFVP PQGSPTMGSS VMGTQMNVIG QSAFGMQGNP FFNPQNFAQP PTTMTNSSSA SNDLKDLFG


    Database link: Q6P3W7

  • Positive control

    • HeLa, NIH 3T3 and MCF7 cell lysates; MCF7 cells; 293 cells, treated with control of SCYL2-siRNA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 50% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    ab221195 is purified by salting-out and ion-exchange chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 104 kDa.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.
  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 HEAT repeat.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    According to PubMed:15809293, autophosphorylated in presence of poly-L-lysine.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes.
  • Information by UniProt
  • Database links

  • Alternative names

    • AU015688 antibody
    • AW536445 antibody
    • BC030932 antibody
    • Coated vesicle-associated kinase of 104 kDa antibody
    • CVAK104 antibody
    • D10Ertd802e antibody
    • FLJ10074 antibody
    • KIAA1360 antibody
    • RGD1305665 antibody
    • SCY1 like 2 (S. cerevisiae) antibody
    • SCY1 like protein 2 antibody
    • SCY1-like protein 2 antibody
    • SCYL2 antibody
    • SCYL2_HUMAN antibody
    see all

Images

  • Immunofluorescent analysis of 4% PFA fixed, 0.25 Triton X-100 permeabilized MCF7 cells labeling SCYL2 with ab221195 at 1/1000 dilution, followed by secondary detection with Alexa Fluor 488 conjugated goat anti rabbit IgG at 1/1000 dilution (green). DAPI was used for nuclear counterstaining (blue). 

  • All lanes : Anti-SCYL2 antibody (ab221195) at 1/1000 dilution

    Lane 1 : 293 cell lysate siRNA control
    Lane 2 : 293 cell lysate treated with SCYL2 siRNA

    Predicted band size: 104 kDa

  • All lanes : Anti-SCYL2 antibody (ab221195) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 104 kDa



    7.5% SDS gel

References

ab221195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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