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    sdf1-antibody-ab18919.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Anti-SDF1 antibody (ab18919)

  • Datasheet
Reviews (1)Q&A (1)References (14)

Product price, shipping and contact information

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Western blot - Anti-SDF1 antibody (ab18919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab18919)

Key features and details

  • Rabbit polyclonal to SDF1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SDF1 antibody
    See all SDF1 primary antibodies
  • Description

    Rabbit polyclonal to SDF1
  • Host species

    Rabbit
  • Specificity

    The antibody should cross-react to both P40224-1 and P40224-2.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Mouse SDF1 aa 1-93. E. coli-expressed mouse SDF1.
    Database link: P40224

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human SDF1 protein (Active) (ab259416)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P
1/25 - 1/50.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P
1/25 - 1/50.

Target

  • Function

    Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • Tissue specificity

    Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Developmental stage

    Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • Post-translational
    modifications

    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P48061 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6387 Human
    • Omim: 600835 Human
    • SwissProt: P48061 Human
    • Unigene: 522891 Human
    • Alternative names

      • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
      • AI174028 antibody
      • C-X-C motif chemokine 12 antibody
      • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
      • Chemokine (C-X-C motif) ligand 12 antibody
      • Chemokine CXC motif ligand 12 antibody
      • cxcl12 antibody
      • hIRH antibody
      • hSDF-1 antibody
      • Intercrine reduced in hepatomas antibody
      • IRH antibody
      • OTTHUMP00000019491 antibody
      • PBSF antibody
      • Pre-B cell growth-stimulating factor antibody
      • SCYB12 antibody
      • SDF 1 antibody
      • SDF-1 antibody
      • SDF-1-alpha(3-67) antibody
      • SDF-1a antibody
      • SDF-1b antibody
      • SDF1_HUMAN antibody
      • SDF1A antibody
      • SDF1B antibody
      • Stromal cell-derived factor 1 antibody
      • Stromal cell-derived factor 1 delta antibody
      • Stromal cell-derived factor 1 gamma antibody
      • Stromal cell-derived factor 1a antibody
      • Stromal cell-derived factor-1 alpha antibody
      • Thymic lymphoma cell-stimulating factor antibody
      • Tlsf antibody
      • TLSF-a antibody
      • TLSF-b antibody
      • Tlsfa antibody
      • Tlsfb antibody
      • TPAR1 antibody
      see all

    Images

    • Western blot - Anti-SDF1 antibody (ab18919)
      Western blot - Anti-SDF1 antibody (ab18919)

      Western blot analysis of SDF-1? expression in transfected cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab18919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab18919)

      ab18919 (2µg/ml) staining SDF1 in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secretory compartments within the smooth muscle fibres.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (14)

    Publishing research using ab18919? Please let us know so that we can cite the reference in this datasheet.

    ab18919 has been referenced in 14 publications.

    • Kurita K  et al. CXCL12-CXCR4 pathway activates brown adipocytes and induces insulin resistance in CXCR4-deficient mice under high-fat diet. Sci Rep 9:6165 (2019). PubMed: 30992469
    • Nicastro M  et al. Fibrocytes in Chronic Periaortitis: A Novel Mechanism Linking Inflammation and Fibrosis. Arthritis Rheumatol 71:1913-1922 (2019). PubMed: 31233292
    • Wu D  et al. The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy. Med Sci Monit 25:6946-6954 (2019). PubMed: 31860633
    • Gimble JM  et al. A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model. Plast Reconstr Surg Glob Open 6:e2010 (2018). PubMed: 30881803
    • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). PubMed: 29335016
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela, Hep G2)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Hela, Hep G2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Question

    Which antigen retrieval protocol should be used with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2006

    Answer

    Thank you for your enquiry. For paraffin-embedded tissue, heat in citrate pH 6.0 buffer for 5 - 10 minutes, heated to boiling either in a microwave or in a steamer. Please do not hesitate to contact us if you have any other questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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