Product nameAnti-SDF1 antibody
See all SDF1 primary antibodies
DescriptionRabbit polyclonal to SDF1
SpecificityThis antibody will react with mouse and rat SDF 1.
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-FoFr, IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Recombinant fragment corresponding to Mouse SDF1 aa 20-89.
Database link: P40224
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab25117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20871610
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 25086214|
|IHC-P||Use at an assay dependent concentration. PubMed: 21224081|
|WB||1/1000. Predicted molecular weight: 11 kDa.|
|IP||1/300 - 1/500.|
FunctionChemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Tissue specificityIsoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Developmental stageIsoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
modificationsProcessed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
- Information by UniProt
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Immunohistochemical analysis of murine epileptic brain tissue, staining SDF1 alpha with ab25117.
Sections were blocked (2% BSA + 10% normal goat serum in 0.1% Triton X-100) for 1 hour at room temperature before being incubated with primary antibody overnight at 4°C. An AlexaFluor®647-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Recombinant mouse SDF1 alpha
Predicted band size: 11 kDa
Detection of recombinant Mouse SDF1 alpha by Western Blot.
ab25117 staining SDF1 alpha in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate pH 6.0. Samples were then blocked with 10% serum for 30 minutes at 25°C and incubated with ab25117 at a 1/100 dilution for 2 hours at 25°C. The secondary used was an undiluted Alexa Fluor® 488-conjugated goat polyclonal.
This product has been referenced in:
- Peyvandi AA et al. Critical role of SDF-1/CXCR4 signaling pathway in stem cell homing in the deafened rat cochlea after acoustic trauma. Neural Regen Res 13:154-160 (2018). Read more (PubMed: 29451220) »
- Pinheiro D et al. Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. PLoS One 12:e0187970 (2017). Read more (PubMed: 29176797) »