Overview

  • Product name
  • Description
    Rabbit polyclonal to SDF1
  • Host species
    Rabbit
  • Specificity
    This antibody will react with mouse and rat SDF 1.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, IHC-FoFr, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment corresponding to Mouse SDF1 aa 20-89.
    Database link: P40224

Properties

Applications

Our Abpromise guarantee covers the use of ab25117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20871610

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 25086214
IHC-P Use at an assay dependent concentration. PubMed: 21224081
WB 1/1000. Predicted molecular weight: 11 kDa.
IP 1/300 - 1/500.

Target

  • Function
    Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • Tissue specificity
    Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Developmental stage
    Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • Post-translational
    modifications
    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
    • AI174028 antibody
    • C-X-C motif chemokine 12 antibody
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
    • Chemokine (C-X-C motif) ligand 12 antibody
    • Chemokine CXC motif ligand 12 antibody
    • cxcl12 antibody
    • hIRH antibody
    • hSDF-1 antibody
    • Intercrine reduced in hepatomas antibody
    • IRH antibody
    • OTTHUMP00000019491 antibody
    • PBSF antibody
    • Pre-B cell growth-stimulating factor antibody
    • SCYB12 antibody
    • SDF 1 antibody
    • SDF-1 antibody
    • SDF-1-alpha(3-67) antibody
    • SDF-1a antibody
    • SDF-1b antibody
    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
    • Stromal cell-derived factor 1a antibody
    • Stromal cell-derived factor-1 alpha antibody
    • Thymic lymphoma cell-stimulating factor antibody
    • Tlsf antibody
    • TLSF-a antibody
    • TLSF-b antibody
    • Tlsfa antibody
    • Tlsfb antibody
    • TPAR1 antibody
    see all

Images

  • Immunohistochemical analysis of murine epileptic brain tissue, staining SDF1 alpha with ab25117.

    Sections were blocked (2% BSA + 10% normal goat serum in 0.1% Triton X-100) for 1 hour at room temperature before being incubated with primary antibody overnight at 4°C. An AlexaFluor®647-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
  • Recombinant mouse SDF1 alpha

    Predicted band size: 11 kDa



    Detection of recombinant Mouse SDF1 alpha by Western Blot.
  • ab25117 staining SDF1 alpha in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate pH 6.0. Samples were then blocked with 10% serum for 30 minutes at 25°C and incubated with ab25117 at a 1/100 dilution for 2 hours at 25°C. The secondary used was an undiluted Alexa Fluor® 488-conjugated goat polyclonal.

    See Abreview

References

This product has been referenced in:
  • Peyvandi AA  et al. Critical role of SDF-1/CXCR4 signaling pathway in stem cell homing in the deafened rat cochlea after acoustic trauma. Neural Regen Res 13:154-160 (2018). Read more (PubMed: 29451220) »
  • Pinheiro D  et al. Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. PLoS One 12:e0187970 (2017). Read more (PubMed: 29176797) »
See all 27 Publications for this product

Customer reviews and Q&As

1-10 of 21 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6,0
Permeabilization
No
Specification
Spleen
Blocking step
BSA+Milk+FBS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 09 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bladder tissue)
Permeabilization
Yes - Triton x100
Specification
Bladder tissue
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 21 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 02 2016

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 29 2016

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - other (muscle)
Specification
muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 04 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (brain tissue)
Specification
brain tissue
Permeabilization
Yes - PBS-Tween 20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 23 2014

Answer


The anti-SDF1 alpha antibody (ab25117) has not been tested for its cross reactivity with samples from chicken however as the homology of the chicken protein and that of the mouse protein (against which this antibody has been raised) only share 75% homology I would not suggest that the antibody will cross react. We do have a different antibody which may be worth trying, this is the rabbit polyclonal ab49124, This has been raised against the region 44-93 of the human SDF1. This shares 86% homology with the chicken protein (Uniprot reference http://www.uniprot.org/uniprot/E2IFI5). This has again not been tested so we would not be able to guarantee that the antibody will cross react.

If you would like to purchase ab49124 and use it with your chicken samples, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab49124 as normal, test the antibody with your samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab49124 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab49124.

Read More

Answer

Thank you for your response, your trust and interest in our products..

I would advise you to look through the available antibodies at this site using the search RabMabs button:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Once you selected the product, please quote the provided promotion code and the antibody code number.

You can place your order via e-mail if you wish by e-mailing us at:orders@abcam.com

If you need any further assistance in the future, please do not hesitate to contact me.

Have a nice weekend!

Read More

Answer

Thank you for your enquiry and your interest in our products.

I can confirm that ab25117 has been tested and characterized in IHC-Fr, Flow Cyt, IHC-P, WB, IP so it works in immunohistochemistry both on frozen and formalin-fixed paraffin-embedded tissue sections and it recognizes mouse and rat SDF1 alpha. The unit pice is €410.00.

You can find some further information about the shipping changes at this site:

https://www.abcam.com/index.html?pageconfig=help#shippingcharges

I would like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused.

I was able to find the recommended protocol for use with CCR2 antibody ab25788. Please note the primary antibody concentration is 1:100. I hope this protocol helps you to achieve good staining with ab25788 anti-CCR2.



Protocol used by lab:

Blocking: 5% BSA for 2hr

Primary antibody: CCR2 antibody- 1:100 in 2.5% BSA (TBS/Tween 0.1%) 1hr at RT

Secondary antibody: Donkey anti-goat HRP - 1:8000 in 2.5% BSA (TBS/Tween 0.1%) 30 min at RT

Visualization: Amersham WB developer

As requested, I have issued a free of charge replacements for the anti-MMP8 and anti-MMP2 antibodies. I have also shipped a B cell lysate for positive control with ab25117 anti-SDF01 alpha. The order number for these is 1184512.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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