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    sdf1-antibody-ab25117.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Anti-SDF1 antibody (ab25117)

  • Datasheet
  • SDS
Reviews (11)Q&A (10)References (46)

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Western blot - Anti-SDF1 antibody (ab25117)

    Key features and details

    • Rabbit polyclonal to SDF1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-SDF1 antibody
      See all SDF1 primary antibodies
    • Description

      Rabbit polyclonal to SDF1
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Recombinant fragment corresponding to Mouse SDF1 aa 20-89.
      Database link: P40224

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Chemokines
      • Alpha Chemokines (CXC)
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Chemokines

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human SDF1 protein (Active) (ab259416)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25117 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Human
    Application Abreviews Notes
    WB (3)
    1/1000. Predicted molecular weight: 11 kDa.
    Notes
    WB
    1/1000. Predicted molecular weight: 11 kDa.

    Target

    • Function

      Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
    • Tissue specificity

      Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Developmental stage

      Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
    • Post-translational
      modifications

      Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P48061 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6387 Human
      • Entrez Gene: 20315 Mouse
      • Omim: 600835 Human
      • SwissProt: P48061 Human
      • SwissProt: P40224 Mouse
      • Unigene: 522891 Human
      • Unigene: 303231 Mouse
      • Alternative names

        • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
        • AI174028 antibody
        • C-X-C motif chemokine 12 antibody
        • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
        • Chemokine (C-X-C motif) ligand 12 antibody
        • Chemokine CXC motif ligand 12 antibody
        • cxcl12 antibody
        • hIRH antibody
        • hSDF-1 antibody
        • Intercrine reduced in hepatomas antibody
        • IRH antibody
        • OTTHUMP00000019491 antibody
        • PBSF antibody
        • Pre-B cell growth-stimulating factor antibody
        • SCYB12 antibody
        • SDF 1 antibody
        • SDF-1 antibody
        • SDF-1-alpha(3-67) antibody
        • SDF-1a antibody
        • SDF-1b antibody
        • SDF1_HUMAN antibody
        • SDF1A antibody
        • SDF1B antibody
        • Stromal cell-derived factor 1 antibody
        • Stromal cell-derived factor 1 delta antibody
        • Stromal cell-derived factor 1 gamma antibody
        • Stromal cell-derived factor 1a antibody
        • Stromal cell-derived factor-1 alpha antibody
        • Thymic lymphoma cell-stimulating factor antibody
        • Tlsf antibody
        • TLSF-a antibody
        • TLSF-b antibody
        • Tlsfa antibody
        • Tlsfb antibody
        • TPAR1 antibody
        see all

      Images

      • Western blot - Anti-SDF1 antibody (ab25117)
        Western blot - Anti-SDF1 antibody (ab25117)
        Recombinant mouse SDF1 alpha

        Predicted band size: 11 kDa



        Detection of recombinant Mouse SDF1 alpha by Western Blot.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (46)

      Publishing research using ab25117? Please let us know so that we can cite the reference in this datasheet.

      ab25117 has been referenced in 46 publications.

      • Sun Z  et al. Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction. Theranostics 10:4967-4982 (2020). PubMed: 32308762
      • Qiu SQ  et al. High hepatocyte growth factor expression in primary tumor predicts better overall survival in male breast cancer. Breast Cancer Res 22:30 (2020). PubMed: 32188473
      • Li G  et al. Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells. J Cell Physiol 234:23485-23494 (2019). PubMed: 31206187
      • Severe N  et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell 25:570-583.e7 (2019). PubMed: 31279774
      • Liu ZY  et al. CXCL12/CXCR4 signaling contributes to neuropathic pain via central sensitization mechanisms in a rat spinal nerve ligation model. CNS Neurosci Ther 25:922-936 (2019). PubMed: 30955244
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 21 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Chicken Tissue sections (Spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH6,0
      Permeabilization
      No
      Specification
      Spleen
      Blocking step
      BSA+Milk+FBS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 09 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SDF1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Bladder tissue)
      Permeabilization
      Yes - Triton x100
      Specification
      Bladder tissue
      Blocking step
      (agent) for 2 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 21 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-SDF1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 02 2016

      Western blot abreview for Anti-SDF1 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain)
      Gel Running Conditions
      Non-reduced Non-Denaturing (Native)
      Loading amount
      30 µg
      Specification
      Brain
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 29 2016

      Western blot abreview for Anti-SDF1 alpha antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      40 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - other (muscle)
      Specification
      muscle
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 04 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SDF1 alpha antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Sample
      Mouse Cell (brain tissue)
      Specification
      brain tissue
      Permeabilization
      Yes - PBS-Tween 20
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 23 2014

      Question

      if there is any information about it, I would like to know whether the rabbit polyclonal anti-SDF-1 alpha antibody (ab25117) has some cross reactivity to chicken.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 25 2013

      Answer


      The anti-SDF1 alpha antibody (ab25117) has not been tested for its cross reactivity with samples from chicken however as the homology of the chicken protein and that of the mouse protein (against which this antibody has been raised) only share 75% homology I would not suggest that the antibody will cross react. We do have a different antibody which may be worth trying, this is the rabbit polyclonal ab49124, This has been raised against the region 44-93 of the human SDF1. This shares 86% homology with the chicken protein (Uniprot reference http://www.uniprot.org/uniprot/E2IFI5). This has again not been tested so we would not be able to guarantee that the antibody will cross react.

      If you would like to purchase ab49124 and use it with your chicken samples, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab49124 as normal, test the antibody with your samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab49124 off any product in our catalogue. More information on this offer can be found here:

      www.abcam.com/abtrial

      Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

      If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab49124.

      Read More

      Abcam Scientific Support

      Answered on Mar 25 2013

      Question

      Thank you very much for the precious information. I've decided to order this antibody (ab25117), and I would like to take also your offer for a free RabMab. How can we indicate in the order which RabMab we choose? And how can we know which antibodies you have still in stock and which ones are instead already finished?
      Thank you very much again,

      Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Dec 07 2012

      Answer

      Thank you for your response, your trust and interest in our products..

      I would advise you to look through the available antibodies at this site using the search RabMabs button:

      https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Once you selected the product, please quote the provided promotion code and the antibody code number.

      You can place your order via e-mail if you wish by e-mailing us at:orders@abcam.com

      If you need any further assistance in the future, please do not hesitate to contact me.

      Have a nice weekend!

      Read More

      Abcam Scientific Support

      Answered on Dec 07 2012

      Question

      To whom it may concern,

      Good afternoon, I would like to ask you some information about your product ab25117, anti-SDF1 alfa antibody, if it is possible:

      .It could be used also for immunohistochemistry experiments?

      .How does it cost, considering also the shipping expenses?

      .Is there available any offer for this product?

      .It could be used for detection of both the isoform SDF1 alfa and beta, in mice?

      Thank you very much in advance,

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Nov 30 2012

      Answer

      Thank you for your enquiry and your interest in our products.

      I can confirm that ab25117 has been tested and characterized in IHC-Fr, Flow Cyt, IHC-P, WB, IP so it works in immunohistochemistry both on frozen and formalin-fixed paraffin-embedded tissue sections and it recognizes mouse and rat SDF1 alpha. The unit pice is €410.00.

      You can find some further information about the shipping changes at this site:

      https://www.abcam.com/index.html?pageconfig=help#shippingcharges

      I would like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

      https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Nov 30 2012

      Question

      Hope you find the following information helpful: MMP8, ab53017, to be replaced by ab81286 MMP2, ab80737, to be replaced by ab92536 SDF-1 alpha, ab25117, awaiting result for refund. Will try mouse B-cell lysate as positive control. I also had really bad result (no signal!) with anti CCR2 antibody, ab25788. Not sure what you can suggest for this one! Thanks for working with me!

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


      I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused.

      I was able to find the recommended protocol for use with CCR2 antibody ab25788. Please note the primary antibody concentration is 1:100. I hope this protocol helps you to achieve good staining with ab25788 anti-CCR2.



      Protocol used by lab:

      Blocking: 5% BSA for 2hr

      Primary antibody: CCR2 antibody- 1:100 in 2.5% BSA (TBS/Tween 0.1%) 1hr at RT

      Secondary antibody: Donkey anti-goat HRP - 1:8000 in 2.5% BSA (TBS/Tween 0.1%) 30 min at RT

      Visualization: Amersham WB developer

      As requested, I have issued a free of charge replacements for the anti-MMP8 and anti-MMP2 antibodies. I have also shipped a B cell lysate for positive control with ab25117 anti-SDF01 alpha. The order number for these is 1184512.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Oct 17 2012

      1-10 of 21 Abreviews or Q&A

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