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  1. Link

    sdf1-antibody-ab9797.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Anti-SDF1 antibody (ab9797)

  • Datasheet
Reviews (14)Q&A (9)References (75)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
  • Sandwich ELISA - Anti-SDF1 antibody (ab9797)
  • Western blot - Anti-SDF1 antibody (ab9797)

Key features and details

  • Rabbit polyclonal to SDF1
  • Suitable for: Sandwich ELISA, WB, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: unknown

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SDF1 antibody [EPR1216] (ab155090)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SDF1 antibody
    See all SDF1 primary antibodies
  • Description

    Rabbit polyclonal to SDF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Goat
  • Immunogen

    Recombinant fragment corresponding to Human SDF1 aa 22-89.
    Sequence:

    KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK


    Database link: P48061
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human lung cancer and human kidney tissues; WB: Recombinant human SDF1 protein (ab9798).
  • General notes

    Although some customers have been successful in IHC we no longer batch test in this application.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Constituent: PBS

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • Recombinant human SDF1 protein (Active) (ab9798)
  • Recombinant Protein

    • Recombinant human SDF1 protein (Active) (ab259416)
  • sELISA pair antibody

    • Biotin Anti-SDF1 antibody (ab84277)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to SDF1 (ab84277). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.
WB
Use at an assay dependent concentration. To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P (8)
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to SDF1 (ab84277). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.
WB
Use at an assay dependent concentration. To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • Tissue specificity

    Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Developmental stage

    Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • Post-translational
    modifications

    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P48061 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6387 Human
    • Omim: 600835 Human
    • SwissProt: P48061 Human
    • Unigene: 522891 Human
    • Alternative names

      • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
      • AI174028 antibody
      • C-X-C motif chemokine 12 antibody
      • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
      • Chemokine (C-X-C motif) ligand 12 antibody
      • Chemokine CXC motif ligand 12 antibody
      • cxcl12 antibody
      • hIRH antibody
      • hSDF-1 antibody
      • Intercrine reduced in hepatomas antibody
      • IRH antibody
      • OTTHUMP00000019491 antibody
      • PBSF antibody
      • Pre-B cell growth-stimulating factor antibody
      • SCYB12 antibody
      • SDF 1 antibody
      • SDF-1 antibody
      • SDF-1-alpha(3-67) antibody
      • SDF-1a antibody
      • SDF-1b antibody
      • SDF1_HUMAN antibody
      • SDF1A antibody
      • SDF1B antibody
      • Stromal cell-derived factor 1 antibody
      • Stromal cell-derived factor 1 delta antibody
      • Stromal cell-derived factor 1 gamma antibody
      • Stromal cell-derived factor 1a antibody
      • Stromal cell-derived factor-1 alpha antibody
      • Thymic lymphoma cell-stimulating factor antibody
      • Tlsf antibody
      • TLSF-a antibody
      • TLSF-b antibody
      • Tlsfa antibody
      • Tlsfb antibody
      • TPAR1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

    • Sandwich ELISA - Anti-SDF1 antibody (ab9797)
      Sandwich ELISA - Anti-SDF1 antibody (ab9797)
      ELISA - Anti-IL-1 beta antibody (ab9797)

      Sandwich ELISA detecting SDF1 using ab9797 at a concentration of 2.0 µg/ml.

    • Western blot - Anti-SDF1 antibody (ab9797)
      Western blot - Anti-SDF1 antibody (ab9797)

      To detect human SDSF1 by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human SDF1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

      Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1, respectively.

      Non-reducing conditions.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (75)

    Publishing research using ab9797? Please let us know so that we can cite the reference in this datasheet.

    ab9797 has been referenced in 75 publications.

    • Ma J  et al. EphA1 Activation Induces Neuropathological Changes in a Mouse Model of Parkinson's Disease Through the CXCL12/CXCR4 Signaling Pathway. Mol Neurobiol 58:913-925 (2021). PubMed: 33057926
    • Zhang X  et al. BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells. Oncogene 40:1516-1530 (2021). PubMed: 33452462
    • Murdamoothoo D  et al. Tenascin-C immobilizes infiltrating T lymphocytes through CXCL12 promoting breast cancer progression. EMBO Mol Med 13:e13270 (2021). PubMed: 33988305
    • Mai CL  et al. CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain. Theranostics 11:1059-1078 (2021). PubMed: 33391521
    • Li S  et al. Bioinformatics analysis identifies biomarkers associated with poor prognosis in diffuse-type gastric cancer. Mol Med Rep 23:N/A (2021). PubMed: 33495829
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    A/Sw/SK/2001 (H1N1) influenza Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate buffer ph 6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20µg/mL · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Dr. manuela martano

    Verified customer

    Submitted Jun 09 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA basic buffer pH 9.0
    Permeabilization
    No
    Specification
    Breast cancer
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Enni Markkanen

    Verified customer

    Submitted Oct 17 2018

    Mass Cytometry abreview for Anti-SDF1 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Mass Cytometry
    Sample
    Mouse Cell (lung)
    Specification
    lung
    Read More

    Irit Milman Krentsis

    Verified customer

    Submitted Feb 20 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Goat Tissue sections (ovary)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Specification
    ovary
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50µg/mL · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Ana Paula Ribeiro Rodrigues

    Verified customer

    Submitted Sep 28 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tooth)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    tooth
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-SDF1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Colon tumor)
    Permeabilization
    Yes - 1x PBS with 0.3% triton-x-100
    Specification
    Colon tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 for 10 min
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    15 min with special blocking serum included in R&D Systems kit as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2014

    ELISA abreview for Anti-SDF1 antibody

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Cell (Supernatants of carcinoma cells)
    Specification
    Supernatants of carcinoma cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Type
    Indirect
    Read More

    DR. Kirsten Hattermann

    Verified customer

    Submitted May 15 2013

    Question

    Testing discount code to try in pig tissue samples.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    DISCOUNT CODE: *
    Expiration date: *

    I am very pleased to hear you would like to accept our offer and test ab9797 in pig tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forpig tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 06 2012

    1-10 of 23 Abreviews or Q&A

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