Recombinant Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)


  • Product name

    Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free
    See all SDF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1216] to SDF1 - Low endotoxin, Azide free
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide corresponding to SDF1.
    Database link: P48061

  • General notes

    ab157772 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab157772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 27220474
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
    • Tissue specificity

      Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Developmental stage

      Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
    • Post-translational

      Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
      • AI174028 antibody
      • C-X-C motif chemokine 12 antibody
      • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
      • Chemokine (C-X-C motif) ligand 12 antibody
      • Chemokine CXC motif ligand 12 antibody
      • cxcl12 antibody
      • hIRH antibody
      • hSDF-1 antibody
      • Intercrine reduced in hepatomas antibody
      • IRH antibody
      • OTTHUMP00000019491 antibody
      • PBSF antibody
      • Pre-B cell growth-stimulating factor antibody
      • SCYB12 antibody
      • SDF 1 antibody
      • SDF-1 antibody
      • SDF-1-alpha(3-67) antibody
      • SDF-1a antibody
      • SDF-1b antibody
      • SDF1_HUMAN antibody
      • SDF1A antibody
      • SDF1B antibody
      • Stromal cell-derived factor 1 antibody
      • Stromal cell-derived factor 1 delta antibody
      • Stromal cell-derived factor 1 gamma antibody
      • Stromal cell-derived factor 1a antibody
      • Stromal cell-derived factor-1 alpha antibody
      • Thymic lymphoma cell-stimulating factor antibody
      • Tlsf antibody
      • TLSF-a antibody
      • TLSF-b antibody
      • Tlsfa antibody
      • Tlsfb antibody
      • TPAR1 antibody
      see all


    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).

    • This ICC data was generated using the same anti-SDF1 antibody clone, EPR1216, in a different buffer formulation (cat# ab155090).

      Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.

    • This ICC data was generated using the same anti-SDF1 antibody clone, EPR1216, in a different buffer formulation (cat# ab155090).

      Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.


    This product has been referenced in:

    • Xu W  et al. Hypoxia changes chemotaxis behaviour of mesenchymal stem cells via HIF-1a signalling. J Cell Mol Med 23:1899-1907 (2019). Read more (PubMed: 30628201) »
    See 1 Publication for this product

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